UseCase15 aphidicolin

Mechanism of DNA Polymerase alpha inhibition by aphidicolin¶ ↑

Context¶ ↑

Aphidicolin,a tetracyclic diterpenoid, is a specific inhibitor of DNA polymerase alpha -{Glover 1984}-. Exposure to aphidicolin results in chromosomal aberrations -{Glover 1984}-.

Mechanism¶ ↑

Aphidicolin is a competitive inhibitor for pol alpha resulting in increased chromosomal aberrations. Aphidicolin forms a complex with DNA polymerase alpha resulting in inhibition of polymerase function causing chromosome gap and break formation -{Glover 1984}-.

Knowledgebase Challenges¶ ↑

Representational
   Biochemical
   Expression(language requirements)

Functional (Question and Answer)

Q. “Which mechanisms of action inhibit DNA polymerase alpha?” A. “Mechanism of DNA Polymerase alpha by aphidicolin” Q. “Which chemicals cause chromosome aberrations?” A. “Aphidicolin”

Process Model¶ ↑

1. Formation of aphidicolin-DNA polymerase alpha complex

2. Inhibition of DNA polymerase alpha activity

3. Induction of chromosome aberrations

‘aphidicolin-DNA polymerase alpha complex’ subClassOf ‘molecular complex’ that ‘has component part’ some ‘aphidicolin’ that ‘has component part’ some ‘DNA polymerase alpha’ ‘aphidicolin-DNA polymerase alpha complex formation’ subClassOf ‘molecular complex formation’ that ‘has target’ some ‘DNA polymerase alpha’ that ‘has target’ some ‘aphidicolin’ and ‘has product’ some ‘aphidicolin-DNA polymerase alpha complex’ ‘inhibition of DNA polymerase alpha by aphidicolin’ subClassof ‘inhibition’ and ‘realizes’ some (‘to inhibit’ that ‘is disposition of’ some (aphidicolin that ‘is component part of’ some ‘aphidicolin-DNA polymerase alpha complex’)) and ‘results in’ some ‘chromosome gap formation’ and ‘results in’ some ‘chromosome breakage’ ‘mechanism of specific inhibition of DNA Polymerase by Aphidicolin’ subClassOf ‘mechanism of specific inhibition of DNA polymerase’ that ‘has part’ some ‘aphidicolin-DNA polymerase alpha complex formation’ that ‘has part’ some ‘inhibition of DNA polymerase alpha by aphidicolin’ and ‘aphidicolin-DNA polymerase alpha complex formation’ and ‘precedes’ some ‘inhibition of DNA polymerase alpha by aphidicolin’

References¶ ↑

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