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feat: enable gzip output for bedtools intersect #3642

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@gtuckerkellogg gtuckerkellogg commented Feb 7, 2025

Enable gzip output for bedtools intersect. Other bedtools to follow.

QC

While the contributions guidelines are more extensive, please particularly ensure that:

  • test.py was updated to call any added or updated example rules in a Snakefile
  • input: and output: file paths in the rules can be chosen arbitrarily
  • wherever possible, command line arguments are inferred and set automatically (e.g. based on file extensions in input: or output:)
  • temporary files are either written to a unique hidden folder in the working directory, or (better) stored where the Python function tempfile.gettempdir() points to
  • the meta.yaml contains a link to the documentation of the respective tool or command under url:
  • conda environments use a minimal amount of channels and packages, in recommended ordering

Summary by CodeRabbit

  • New Features

    • Enabled automatic gzip compression for output files in various genomic analysis commands when filenames end with ".gz".
    • Added a new workflow rule to generate compressed intersection results, improving output management.
  • Tests

    • Expanded test coverage with a new scenario verifying the handling of compressed intersection outputs.
    • Updated the sorting test with an additional flag to ensure consistent command execution.

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coderabbitai bot commented Feb 7, 2025

📝 Walkthrough

Walkthrough

The changes add conditional logic across multiple bedtools wrapper scripts to detect if the output filename ends with ".gz" and, if so, append a gzip compression pipe to the command execution. Additionally, a new Snakefile rule for generating gzipped intersect outputs has been introduced along with corresponding test updates to cover the new functionality and enhance flag usage.

Changes

File(s) Change Summary
bio/bedtools/bamtobed/wrapper.py
bio/bedtools/complement/wrapper.py
bio/bedtools/coveragebed/wrapper.py
bio/bedtools/genomecov/wrapper.py
bio/bedtools/intersect/wrapper.py
bio/bedtools/merge/wrapper.py
Added a conditional check on the output filename extension; if it ends with ".gz", the shell command is modified by appending "| gzip" to enable gzip compression.
bio/bedtools/intersect/test/Snakefile Added a new rule, "bedtools_merge_zip", mirroring the existing merge rule but configured to produce a gzipped output ("A_B.intersected.bed.gz").
test_wrappers.py Introduced a new test function, "test_bedtools_intersect_gzip", to validate the gzipped output scenario, and updated "test_bedtools_sort" to include the "-F" flag for improved command execution.

Sequence Diagram(s)

sequenceDiagram
    participant S as Snakemake
    participant W as Wrapper Script
    participant Sh as Shell
    S->>W: Execute command for bedtools tool
    Note over W: Check if output filename ends with ".gz"
    alt Output requires compression
        W->>W: Set compress variable to "| gzip"
    else
        W->>W: Set compress variable to empty string
    end
    W->>Sh: Run shell command (with optional gzip)
    Sh-->>W: Return result
    W-->>S: Output generated file
Loading
sequenceDiagram
    participant SF as Snakefile Rule (bedtools_merge_zip)
    participant W as Intersect Wrapper
    participant Sh as Shell
    SF->>W: Call intersect wrapper
    Note over W: Include compression logic based on output extension
    W->>Sh: Execute command with optional "| gzip"
    Sh-->>W: Return execution result
    W-->>SF: Produce gzipped output file
Loading

Suggested reviewers

  • fgvieira
  • johanneskoester
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@gtuckerkellogg gtuckerkellogg marked this pull request as draft February 7, 2025 03:49
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Actionable comments posted: 0

🧹 Nitpick comments (3)
bio/bedtools/bamtobed/wrapper.py (1)

12-15: LGTM! Compression logic is correctly implemented.

The conditional check for .gz extension and the corresponding compression command setup is accurate.

Optional: Consider using a more concise ternary operator:

-if snakemake.output[0].endswith(".gz"):
-    compress = "| gzip"
-else:
-    compress = ""
+compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""
🧰 Tools
🪛 Ruff (0.8.2)

12-15: Use ternary operator compress = "| gzip" if snakemake.output[0].endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""

(SIM108)


12-12: Undefined name snakemake

(F821)

bio/bedtools/complement/wrapper.py (1)

10-13: LGTM! Compression logic is correctly implemented.

The conditional check for .gz extension and the corresponding compression command setup is accurate.

Optional: Consider using a more concise ternary operator:

-if snakemake.output[0].endswith(".gz"):
-    compress = "| gzip"
-else:
-    compress = ""
+compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""
🧰 Tools
🪛 Ruff (0.8.2)

10-13: Use ternary operator compress = "| gzip" if snakemake.output[0].endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""

(SIM108)


10-10: Undefined name snakemake

(F821)

bio/bedtools/intersect/wrapper.py (1)

12-16: LGTM! Well-documented compression logic.

The compression feature is well-documented and correctly implemented.

Optional: Consider using a more concise ternary operator:

-if snakemake.output[0].endswith(".gz"):
-    compress = "| gzip"
-else:
-    compress = ""
+compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""
🧰 Tools
🪛 Ruff (0.8.2)

13-16: Use ternary operator compress = "| gzip" if snakemake.output[0].endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""

(SIM108)


13-13: Undefined name snakemake

(F821)

📜 Review details

Configuration used: CodeRabbit UI
Review profile: CHILL
Plan: Pro

📥 Commits

Reviewing files that changed from the base of the PR and between b84196c and 9c5f406.

📒 Files selected for processing (8)
  • bio/bedtools/bamtobed/wrapper.py (1 hunks)
  • bio/bedtools/complement/wrapper.py (1 hunks)
  • bio/bedtools/coveragebed/wrapper.py (2 hunks)
  • bio/bedtools/genomecov/wrapper.py (1 hunks)
  • bio/bedtools/intersect/test/Snakefile (1 hunks)
  • bio/bedtools/intersect/wrapper.py (1 hunks)
  • bio/bedtools/merge/wrapper.py (2 hunks)
  • test_wrappers.py (2 hunks)
🧰 Additional context used
📓 Path-based instructions (2)
`**/*.py`: Do not try to improve formatting. Do not suggest ...

**/*.py: Do not try to improve formatting.
Do not suggest type annotations for functions that are defined inside of functions or methods.
Do not suggest type annotation of the self argument of methods.
Do not suggest type annotation of the cls argument of classmethods.
Do not suggest return type annotation if a function or method does not contain a return statement.

  • bio/bedtools/coveragebed/wrapper.py
  • bio/bedtools/merge/wrapper.py
  • bio/bedtools/intersect/wrapper.py
  • bio/bedtools/complement/wrapper.py
  • bio/bedtools/bamtobed/wrapper.py
  • bio/bedtools/genomecov/wrapper.py
  • test_wrappers.py
`**/wrapper.py`: Do not complain about use of undefined vari...

**/wrapper.py: Do not complain about use of undefined variable called snakemake.

  • bio/bedtools/coveragebed/wrapper.py
  • bio/bedtools/merge/wrapper.py
  • bio/bedtools/intersect/wrapper.py
  • bio/bedtools/complement/wrapper.py
  • bio/bedtools/bamtobed/wrapper.py
  • bio/bedtools/genomecov/wrapper.py
🪛 Ruff (0.8.2)
bio/bedtools/coveragebed/wrapper.py

20-23: Use ternary operator compress = "| gzip" if output_file.endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if output_file.endswith(".gz") else ""

(SIM108)

bio/bedtools/merge/wrapper.py

13-16: Use ternary operator compress = "| gzip" if snakemake.output[0].endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""

(SIM108)


13-13: Undefined name snakemake

(F821)

bio/bedtools/intersect/wrapper.py

13-16: Use ternary operator compress = "| gzip" if snakemake.output[0].endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""

(SIM108)


13-13: Undefined name snakemake

(F821)

bio/bedtools/complement/wrapper.py

10-13: Use ternary operator compress = "| gzip" if snakemake.output[0].endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""

(SIM108)


10-10: Undefined name snakemake

(F821)


16-16: Undefined name snakemake

(F821)

bio/bedtools/bamtobed/wrapper.py

12-15: Use ternary operator compress = "| gzip" if snakemake.output[0].endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""

(SIM108)


12-12: Undefined name snakemake

(F821)

bio/bedtools/genomecov/wrapper.py

22-25: Use ternary operator compress = "| gzip" if snakemake.output[0].endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""

(SIM108)


22-22: Undefined name snakemake

(F821)

⏰ Context from checks skipped due to timeout of 90000ms (1)
  • GitHub Check: Summary
🔇 Additional comments (13)
bio/bedtools/bamtobed/wrapper.py (1)

23-23: LGTM! Shell command is correctly modified.

The compression command is properly placed in the shell command before the output redirection.

bio/bedtools/complement/wrapper.py (1)

23-23: LGTM! Shell command is correctly modified.

The compression command is properly placed in the shell command before the output redirection.

bio/bedtools/intersect/wrapper.py (1)

23-23: LGTM! Shell command is correctly modified.

The compression command is properly placed in the shell command before the output redirection.

bio/bedtools/intersect/test/Snakefile (1)

16-28: Consider using a unique log file for the gzipped output rule.

The new rule is well-structured and correctly implements gzipped output. However, both rules are using the same log file path which could lead to log overwrites if both rules are executed in the same workflow.

Consider using a unique log file path for the gzipped output rule:

     log:
-        "logs/intersect/A_B.log"
+        "logs/intersect/A_B.gz.log"
✅ Verification successful

Unique log file needed for gzipped output rule

The current implementation in rule bedtools_merge_zip is using the log file "logs/intersect/A_B.log", which is shared with another rule. This introduces the possibility of log overwrites when both rules run in the same workflow. Changing the log file to a unique name (e.g., "logs/intersect/A_B.gz.log") for the gzipped output rule—as suggested—is appropriate.

  • In bio/bedtools/intersect/test/Snakefile (lines 16–28), change the log file path to "logs/intersect/A_B.gz.log".
bio/bedtools/coveragebed/wrapper.py (2)

20-26: LGTM! Compression detection logic is correctly implemented.

The implementation correctly detects if the output file should be compressed based on the .gz extension.

🧰 Tools
🪛 Ruff (0.8.2)

20-23: Use ternary operator compress = "| gzip" if output_file.endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if output_file.endswith(".gz") else ""

(SIM108)


35-35: LGTM! Compression pipe is correctly placed.

The compression pipe is correctly placed before the output redirection, ensuring proper handling of gzipped output.

bio/bedtools/genomecov/wrapper.py (2)

22-26: LGTM! Compression detection logic is correctly implemented.

The implementation correctly detects if the output file should be compressed based on the .gz extension.

🧰 Tools
🪛 Ruff (0.8.2)

22-25: Use ternary operator compress = "| gzip" if snakemake.output[0].endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""

(SIM108)


22-22: Undefined name snakemake

(F821)


32-32: LGTM! Compression pipe is correctly placed.

The compression pipe is correctly placed before the output redirection, ensuring proper handling of gzipped output.

bio/bedtools/merge/wrapper.py (3)

13-18: LGTM! Compression detection logic is correctly implemented.

The implementation correctly detects if the output file should be compressed based on the .gz extension.

🧰 Tools
🪛 Ruff (0.8.2)

13-16: Use ternary operator compress = "| gzip" if snakemake.output[0].endswith(".gz") else "" instead of if-else-block

Replace if-else-block with compress = "| gzip" if snakemake.output[0].endswith(".gz") else ""

(SIM108)


13-13: Undefined name snakemake

(F821)


30-32: LGTM! Compression pipe is correctly placed for multiple input files.

The compression pipe is correctly placed before the output redirection when handling multiple input files.


40-41: LGTM! Compression pipe is correctly placed for single input file.

The compression pipe is correctly placed before the output redirection when handling a single input file.

test_wrappers.py (2)

1957-1957: LGTM! Appropriate flag addition.

The addition of the -F flag ensures consistent behavior with force overwrite.


1984-1989: LGTM! Good test coverage for gzip output functionality.

The new test function properly verifies the gzip output capability for bedtools intersect.

Let's verify that the test covers all key aspects of the gzip functionality:

✅ Verification successful

🏁 Script executed:

#!/bin/bash
# Description: Check if the test covers all necessary aspects of gzip output

# Check if the corresponding wrapper handles gzip detection and compression
ast-grep --pattern $'if output.endswith(".gz"):
  $$$
  "| gzip"
  $$$'

Length of output: 72


I'll run an additional script to inspect the new test function in "test_wrappers.py" and check for any gzip handling logic in the "bio/bedtools/intersect" wrapper. This should help us verify that the test indeed exercises the gzip-related functionality.


🏁 Script executed:

#!/bin/bash
echo "Examining the new test function in test_wrappers.py (lines 1980-1995):"
sed -n '1980,1995p' test_wrappers.py

echo ""
echo "Searching for gzip-related logic in the bedtools intersect wrapper (bio/bedtools/intersect):"
rg "\.gz" bio/bedtools/intersect -n

Length of output: 986


LGTM: The new test function properly exercises the gzip-specific functionality.

• The new test calls the wrapper with an output filename ending in ".bed.gz", and the wrapper confirms gzip handling by checking for ".gz" in the output.
• The additional "-F" flag in other tests remains consistent with expected behavior.

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