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SpatialPlot issue: spot sizes within the slices are inconsistent #9491

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onmyojiyys opened this issue Nov 18, 2024 · 1 comment
Open

SpatialPlot issue: spot sizes within the slices are inconsistent #9491

onmyojiyys opened this issue Nov 18, 2024 · 1 comment
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@onmyojiyys
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Hi Seurat team,

When using the SpatialPlot function in Seurat 5.1.0 to generate images, I find that the spot sizes within the slices are inconsistent, how can this be resolved?
p <- SpatialPlot(seurat_object, features = 'nCount_Spatial', ncol = 1, pt.size.factor=1.6, crop=FALSE, image.alpha=0.4, shape = 22)
image

When I set the pt.size.factor parameter to 1, this phenomenon becomes more pronounced.

image

@onmyojiyys onmyojiyys added the bug Something isn't working label Nov 18, 2024
@zskylarli
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Hi - thanks for reporting this issue, do you know if this happens when you do not use squares as the shape (i.e. shape = 21 instead)?

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