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34 changes: 34 additions & 0 deletions CITATION.cff
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# This CITATION.cff file was generated with cffinit.
# Visit https://bit.ly/cffinit to generate yours today!

cff-version: 1.2.0
title: >-
scCRISPR-seq Perturbation Analysis Workflow using Seurat's
Mixscape
message: >-
If you use this software, please cite it using the
metadata from this file.
type: software
authors:
- given-names: Stephan
family-names: Reichl
orcid: 'https://orcid.org/0000-0001-8555-7198'
affiliation: CeMM Research Center for Molecular Medicine
- given-names: Christoph
family-names: Bock
orcid: 'https://orcid.org/0000-0001-6091-3088'
affiliation: CeMM Research Center for Molecular Medicine
repository-code: 'https://github.com/epigen/mixscape_seurat'
url: 'https://epigen.github.io/mixscape_seurat/'
abstract: >-
A Snakemake workflow for performing perturbation analyses
of pooled (multimodal) CRISPR screens with sc/snRNA-seq
read-out (scCRISPR-seq) using the R package Seurat's
method Mixscape.
keywords:
- scCRISPR-seq
- Perturbation Analysis
- Bioinformatics
- Workflow
- Snakemake
license: MIT
11 changes: 11 additions & 0 deletions README.md
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# scCRISPR-seq Perturbation Analysis Snakemake Workflow powered by Seurat's Mixscape
A [Snakemake](https://snakemake.readthedocs.io/en/stable/) workflow for performing perturbation analyses of pooled (multimodal) CRISPR screens with sc/snRNA-seq read-out (scCRISPR-seq) powered by the R package [Seurat's](https://satijalab.org/seurat/index.html) method [Mixscape](https://satijalab.org/seurat/articles/mixscape_vignette.html).

This workflow adheres to the module specifications of [MR.PARETO](https://github.com/epigen/mr.pareto), an effort to augment research by modularizing (biomedical) data science. For more details and modules check out the project's repository.

**If you use this workflow in a publication, don't forget to give credits to the authors by citing the URL of this (original) repository (and its DOI, see Zenodo badge above -> coming soon).**

![Workflow Rulegraph](./workflow/dags/rulegraph.svg)
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* [Configuration](#configuration)
* [Example](#example)
* [Links](#links)
* [Publications](#publications)

# Authors
- [Stephan Reichl](https://github.com/sreichl)
- [Christoph Bock](https://github.com/chrbock)


# Software
This project wouldn't be possible without the following software and it's dependencies:

| Software | Reference (DOI) |
| :------------: | :-----------------------------------------------: |
| ggplot2 | https://ggplot2.tidyverse.org/ |
| Mixscape | https://doi.org/10.1038/s41588-021-00778-2 |
| mixtools | https://CRAN.R-project.org/package=mixtools |
| patchwork | https://CRAN.R-project.org/package=patchwork |
| Seurat | https://doi.org/10.1016/j.cell.2021.04.048 |
| Snakemake | https://doi.org/10.12688/f1000research.29032.2 |

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- [GitHub Page](https://epigen.github.io/mixscape_seurat/)
- [Zenodo Repository (coming soon)]()
- [Snakemake Workflow Catalog Entry](https://snakemake.github.io/snakemake-workflow-catalog?usage=epigen/mixscape_seurat)

# Publications
The following publications successfully used this module for their analyses.
- ...

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