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{"name":"minimap2","description":"minimap2","usage":"minimap2 [options] <target.fa>|<target.idx> [query.fa] [...]","options":[{"names":["-x"],"argument":"{map-pb|map_ont|map-hifi|ava-pb|ava-ont|asm5|asm10|asm20|splice|splice:hg|sr}","description":"preset (always applied before other options; see minimap2.1 for details) []"},{"names":["-H"],"argument":"","description":"use homopolymer-compressed k-mer (preferrable for PacBio)"},{"names":["-k"],"argument":"INT","description":"k-mer size (no larger than 28) [15]"},{"names":["-w"],"argument":"INT","description":"minimizer window size [10]"},{"names":["-I"],"argument":"NUM","description":"split index for every ~NUM input bases [4G]"},{"names":["-d"],"argument":"FILE","description":"dump index to FILE []"},{"names":["-f"],"argument":"FLOAT","description":"filter out top FLOAT fraction of repetitive minimizers [0.0002]"},{"names":["-g"],"argument":"NUM","description":"stop chain enlongation if there are no minimizers in INT-bp [5000]"},{"names":["-G"],"argument":"NUM","description":"max intron length (effective with -xsplice; changing -r) [200k]"},{"names":["-F"],"argument":"NUM","description":"max fragment length (effective with -xsr or in the fragment mode) [800]"},{"names":["-r"],"argument":"NUM[,NUM]","description":"chaining/alignment bandwidth and long-join bandwidth [500,20000]"},{"names":["-n"],"argument":"INT","description":"minimal number of minimizers on a chain [3]"},{"names":["-m"],"argument":"INT","description":"minimal chaining score (matching bases minus log gap penalty) [40]"},{"names":["-X"],"argument":"","description":"skip self and dual mappings (for the all-vs-all mode)"},{"names":["-p"],"argument":"FLOAT","description":"min secondary-to-primary score ratio [0.8]"},{"names":["-N"],"argument":"INT","description":"retain at most INT secondary alignments [5]"},{"names":["-A"],"argument":"INT","description":"matching score [2]"},{"names":["-B"],"argument":"INT","description":"mismatch penalty [4]"},{"names":["-O"],"argument":"INT[,INT]","description":"gap open penalty [4,24]"},{"names":["-E"],"argument":"INT[,INT]","description":"gap extension penalty; a k-long gap costs min{O1+k*E1,O2+k*E2} [2,1]"},{"names":["-z"],"argument":"INT[,INT]","description":"Z-drop score and inversion Z-drop score [400,200]"},{"names":["-s"],"argument":"INT","description":"minimal peak DP alignment score [80]"},{"names":["-u"],"argument":"CHAR","description":"how to find GT-AG. f:transcript strand, b:both strands, n:don't match GT-AG [n]"},{"names":["-a"],"argument":"","description":"output in the SAM format (PAF by default)"},{"names":["-o"],"argument":"FILE","description":"output alignments to FILE [stdout]"},{"names":["-L"],"argument":"","description":"write CIGAR with >65535 ops at the CG tag"},{"names":["-R"],"argument":"STR","description":"SAM read group line in a format like '@RG\\tID:foo\\tSM:bar' []"},{"names":["-c"],"argument":"","description":"output CIGAR in PAF"},{"names":["--cs"],"argument":"[=STR]","description":"output the cs tag; STR is 'short' (if absent) or 'long' [none]"},{"names":["--MD"],"argument":"","description":"output the MD tag"},{"names":["--eqx"],"argument":"","description":"write =/X CIGAR operators"},{"names":["-Y"],"argument":"","description":"use soft clipping for supplementary alignments"},{"names":["-t"],"argument":"INT","description":"number of threads [3]"},{"names":["-K"],"argument":"NUM","description":"minibatch size for mapping [500M]"},{"names":["--version"],"argument":"","description":"show version number"}],"version":"2.24-r1122","tldr":"> Map and align between collections of DNA sequences.\n> More information: <https://lh3.github.io/minimap2/>.\n\n- Get alignment in SAM format\n\n`minimap2 -a {{ref.fa}} {{query.fq}} > {{alignment.sam}}`\n\n- Map long noisy genomic reads (PacBio):\n\n`minimap2 -ax map-pb {{ref.fa}} {{pacbio-reads.fq}} > {{aln.sam}}`\n\n- Map long cDNA-seq reads (Oxford Nanopore):\n\n`minimap2 -ax splice {{ref.fa}} {{nanopore-cdna.fa}} > {{aln.sam}}`\n\n- Find overlaps between long reads (Oxford Nanopore):\n\n`minimap2 -x ava-ont {{reads.fq}} {{reads.fq}} > {{ovlp.paf}}`\n\n- Map short accurate paired-end genomic reads:\n\n`minimap2 -ax sr {{ref.fa}} {{read1.fq}} {{read2.fq}} > s{{aln.sam}}`\n\n- Full genome/assembly alignment:\n\n`minimap2 -ax asm5 {{ref.fa}} {{asm.fa}} > {{aln.sam}}`\n"} | ||
{"name":"minimap2","description":"minimap2","usage":"minimap2 [options] <target.fa>|<target.idx> [query.fa] [...]","options":[{"names":["-x"],"argument":"{map-ont|lr:hq|map-hifi|map-pb|map-iclr|asm5|asm10|asm20|splice|splice:hg|sr|ava-pb|ava-ont}","description":"preset (always applied before other options; see minimap2.1 for details) []"},{"names":["-H"],"argument":"","description":"use homopolymer-compressed k-mer (preferrable for PacBio)"},{"names":["-k"],"argument":"INT","description":"k-mer size (no larger than 28) [15]"},{"names":["-w"],"argument":"INT","description":"minimizer window size [10]"},{"names":["-I"],"argument":"NUM","description":"split index for every ~NUM input bases [8G]"},{"names":["-d"],"argument":"FILE","description":"dump index to FILE []"},{"names":["-f"],"argument":"FLOAT","description":"filter out top FLOAT fraction of repetitive minimizers [0.0002]"},{"names":["-g"],"argument":"NUM","description":"stop chain enlongation if there are no minimizers in INT-bp [5000]"},{"names":["-G"],"argument":"NUM","description":"max intron length (effective with -xsplice; changing -r) [200k]"},{"names":["-F"],"argument":"NUM","description":"max fragment length (effective with -xsr or in the fragment mode) [800]"},{"names":["-r"],"argument":"NUM[,NUM]","description":"chaining/alignment bandwidth and long-join bandwidth [500,20000]"},{"names":["-n"],"argument":"INT","description":"minimal number of minimizers on a chain [3]"},{"names":["-m"],"argument":"INT","description":"minimal chaining score (matching bases minus log gap penalty) [40]"},{"names":["-X"],"argument":"","description":"skip self and dual mappings (for the all-vs-all mode)"},{"names":["-p"],"argument":"FLOAT","description":"min secondary-to-primary score ratio [0.8]"},{"names":["-N"],"argument":"INT","description":"retain at most INT secondary alignments [5]"},{"names":["-A"],"argument":"INT","description":"matching score [2]"},{"names":["-B"],"argument":"INT","description":"mismatch penalty (larger value for lower divergence) [4]"},{"names":["-O"],"argument":"INT[,INT]","description":"gap open penalty [4,24]"},{"names":["-E"],"argument":"INT[,INT]","description":"gap extension penalty; a k-long gap costs min{O1+k*E1,O2+k*E2} [2,1]"},{"names":["-z"],"argument":"INT[,INT]","description":"Z-drop score and inversion Z-drop score [400,200]"},{"names":["-s"],"argument":"INT","description":"minimal peak DP alignment score [80]"},{"names":["-u"],"argument":"CHAR","description":"how to find GT-AG. f:transcript strand, b:both strands, n:don't match GT-AG [n]"},{"names":["-J"],"argument":"INT","description":"splice mode. 0: original minimap2 model; 1: miniprot model [1]"},{"names":["-a"],"argument":"","description":"output in the SAM format (PAF by default)"},{"names":["-o"],"argument":"FILE","description":"output alignments to FILE [stdout]"},{"names":["-L"],"argument":"","description":"write CIGAR with >65535 ops at the CG tag"},{"names":["-R"],"argument":"STR","description":"SAM read group line in a format like '@RG\\tID:foo\\tSM:bar' []"},{"names":["-c"],"argument":"","description":"output CIGAR in PAF"},{"names":["--cs"],"argument":"[=STR]","description":"output the cs tag; STR is 'short' (if absent) or 'long' [none]"},{"names":["--ds"],"argument":"","description":"output the ds tag, which is an extension to cs"},{"names":["--MD"],"argument":"","description":"output the MD tag"},{"names":["--eqx"],"argument":"","description":"write =/X CIGAR operators"},{"names":["-Y"],"argument":"","description":"use soft clipping for supplementary alignments"},{"names":["-t"],"argument":"INT","description":"number of threads [3]"},{"names":["-K"],"argument":"NUM","description":"minibatch size for mapping [500M]"},{"names":["--version"],"argument":"","description":"show version number"}],"version":"2.28-r1209","tldr":"> Map and align between collections of DNA sequences.\n> More information: <https://lh3.github.io/minimap2/>.\n\n- Get alignment in SAM format\n\n`minimap2 -a {{ref.fa}} {{query.fq}} > {{alignment.sam}}`\n\n- Map long noisy genomic reads (PacBio):\n\n`minimap2 -ax map-pb {{ref.fa}} {{pacbio-reads.fq}} > {{aln.sam}}`\n\n- Map long cDNA-seq reads (Oxford Nanopore):\n\n`minimap2 -ax splice {{ref.fa}} {{nanopore-cdna.fa}} > {{aln.sam}}`\n\n- Find overlaps between long reads (Oxford Nanopore):\n\n`minimap2 -x ava-ont {{reads.fq}} {{reads.fq}} > {{ovlp.paf}}`\n\n- Map short accurate paired-end genomic reads:\n\n`minimap2 -ax sr {{ref.fa}} {{read1.fq}} {{read2.fq}} > s{{aln.sam}}`\n\n- Full genome/assembly alignment:\n\n`minimap2 -ax asm5 {{ref.fa}} {{asm.fa}} > {{aln.sam}}`\n"} |
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