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So I have some bulkRNA samples I want to check are actually from the donors they are assigned to. To test this, I created a vcf file (using cellSNP-lite) where I have seven bulk samples from two donors each. The samples are from two different tissues from different time points.
When I use the -e 0 flag, and order based on discordance from lower to higher, I get the pairs from different donors as the lowest discordance and pairs from the same donor with the highest discordance. Without the -e 0 flag sorting the same way i get first some mismatches, then all of the matching pairs and at the end most of the mismatched pairs. Is there something wrong with the tool, am I running something wrong or am I not interpreting the results properly?
So I have some bulkRNA samples I want to check are actually from the donors they are assigned to. To test this, I created a vcf file (using cellSNP-lite) where I have seven bulk samples from two donors each. The samples are from two different tissues from different time points.
When I use the -e 0 flag, and order based on discordance from lower to higher, I get the pairs from different donors as the lowest discordance and pairs from the same donor with the highest discordance. Without the -e 0 flag sorting the same way i get first some mismatches, then all of the matching pairs and at the end most of the mismatched pairs. Is there something wrong with the tool, am I running something wrong or am I not interpreting the results properly?
Here's the list of samples for both donors
donor 1
5594
A471
A472
A473
A652
C686
F948
donor 5
9179
A458
A476
A477
A654
C689
F950
merged_1_5_output.txt
merged_1_5_output_e0.txt
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