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Pipeline succeeded only for the first sample in the sample sheet #188

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leurwu opened this issue Mar 6, 2025 · 1 comment
Open

Pipeline succeeded only for the first sample in the sample sheet #188

leurwu opened this issue Mar 6, 2025 · 1 comment
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@leurwu
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leurwu commented Mar 6, 2025

Description of the bug

I’m trying out the dev version on my sample sets and encountered a problem. My sample sheet has 20 samples and the pipeline only ran the whole process on the first sample, for the remaining 19 samples they only finished the FastQC step. The pipeline was still reported as completed successfully.

nextflow.log

Command used and terminal output

nextflow run nf-core/rnavar -r dev \
                --input /home/hpcngoc1/storage/gatk_rnaseq/samplesheet_batch1.csv \
                --fasta /rds/project/who1000-1/rds-who1000-cbrc/data/NBR-RNAseq/software/reference_files/ensembl/fasta_genome/Homo_sapiens.GRCh38.dna_sm.primary_assembly.fa \
                --gtf /rds/project/who1000-1/rds-who1000-cbrc/data/NBR-RNAseq/software/reference_files/ensembl/gtf/Homo_sapiens.GRCh38.112.gtf \
                --star_index /rds/project/who1000-1/rds-who1000-cbrc/data/NBR-RNAseq/software/reference_files//ensembl/genome_indexed_star2.7.4a \
                --dict /rds/project/who1000-1/rds-who1000-cbrc/data/NBR-RNAseq/software/reference_files/ensembl/genome_indexed_star2.7.4a/genome/Homo_sapiens.GRCh38.dna_sm.primary_assembly.dict \
                --fasta_fai /rds/project/who1000-1/rds-who1000-cbrc/data/NBR-RNAseq/software/reference_files//ensembl/genome_indexed_star2.7.4a/genome/Homo_sapiens.GRCh38.dna_sm.primary_assembly.fa.fai \
                --read_length 100 --dbsnp /rds/project/who1000-1/rds-who1000-cbrc/data/NBR-RNAseq/software/reference_files//rnavar/dbsnp_00-common_all.vcf.gz \
                --dbsnp_tbi /rds/project/who1000-1/rds-who1000-cbrc/data/NBR-RNAseq/software/reference_files//rnavar/dbsnp_00-common_all.vcf.gz.tbi \
                --skip_variantannotation \
                --outdir /home/hpcngoc1/storage/gatk_rnaseq/out_batch1 \
                --partition cclake --project BRIDGE-OLD-SL2-CPU \
                -work-dir /home/hpcngoc1/storage/gatk_rnaseq/work -profile cambridge -resume

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@leurwu leurwu added the bug Something isn't working label Mar 6, 2025
@maxulysse
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@leurwu can you try with -r dev -latest

@maxulysse maxulysse added this to the 1.2.0 milestone Mar 10, 2025
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