-
Notifications
You must be signed in to change notification settings - Fork 14
Forgot to save unmapped reads?
Serghei Mangul edited this page Aug 7, 2017
·
1 revision
Forgot to save unmapped reads, we got you covered. Use --digGold
and -a
options
For example:
python imrep.py --digGold -a example/toyExample_allReads.fastq example/toyExample_onlyMapped.bam example/toyExample.cdr3
This is expected output
Parse fastq file with orignal raw reads (all reads) and extract the unmapped reads
Number of reads extacted from IGH locus : 0
Number of reads extacted from IGK locus : 0
Number of reads extacted from IGL locus : 0
Number of reads extacted from TRA locus : 0
Number of reads extacted from TRB locus : 0
Number of reads extacted from TRD locus : 0
Number of reads extacted from TRG locus : 0
Parse bam file with mapped reads
Number of unmapped reads extracted 100
Starting ImReP-0.6 (developped by Igor Mandric, Serghei Mangul)
_
(_)_ __ ___ _ __ ___ _ __
| | '_ ` _ \| '__/ _ \ '_ \
| | | | | | | | | __/ |_) |
|_|_| |_| |_|_| \___| .__/
|_|
5 partial-V CDR3 found
0 partial-J CDR3 found
8 full CDR3 found:
- 8 of type IGH
- 0 of type IGK
- 0 of type IGL
- 0 of type TRA
- 0 of type TRB
- 0 of type TRD
- 0 of type TRG
Done. Bye-bye