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fulcrumgenomics · Nov 15, 2024 · e503bf6 · e503bf6
1 parent febbefb
commit e503bf6
Showing 1 changed file with 2 additions and 0 deletions.
diff --git a/prymer/api/picking.py b/prymer/api/picking.py
@@ -146,6 +146,8 @@ def build_primer_pairs(
                 if amp_span.length > amplicon_sizes.max:
                     continue
 
+                # Since the amplicon span and the region_start are both 1-based, the minuend
+                # becomes a zero-based offset
                 amp_bases = bases[amp_span.start - region_start : amp_span.end - region_start + 1]
                 amp_tm = calculate_long_seq_tm(amp_bases)