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@BioWilko BioWilko released this 13 Nov 13:26
· 24 commits to master since this release

ARTIC Fieldbioinformatics v1.5.1

Well hasn't this been a long time coming?

Major Changes:

  • Fieldbioinformatics now supports rapid barcoded (fragmented) primer trimming and normalisation
  • Nanopolish has been removed completely due to several compatibility issues
  • Medaka has also been removed completely due to kicking out long indels in a way that cannot be changed.
  • Clair3 is now the default variant caller, by default only the r9.4.1 models are available but a artic_get_models command has been added which will fetch the ONT created r10.4.1 models listed in the rerio repository.
  • The pipeline will also attempt to pick an appropriate model based on the basecall_model_version_id field that is added to read headers by default by ONT sequencers.
  • Removed longshot entirely, it also kicks out long variants and is now unnecessary due to clair3 being a much better variant caller.
  • Primer scheme fetcher has been updated to pull from the quick-lab primal hub schemes repository. For schemes not available in this repository you may provide them directly with the arguments --bed and --ref.
  • Automated docker builds pushing to quay.io for use in nextflow pipelines etc.
  • Remove some old functionality which is no longer relevant (basecalling, gather, etc)
  • Re-implement CI as a gh action.
  • Fix the overlapping variants issue by normalising variants against the pre-consensus using bcftools norm.

What's Changed

Full Changelog: v1.2.4...v1.5.1