R Scripts used in scRNA-seq analysis of ND and T2D Islets
These can be found in the master branch.
Packages used came from Bioconductor: Amezquita, R. A. et al. (2020) Orchestrating single-cell analysis with Bioconductor, Nature Methods, 17(2), pp. 137–145. doi: 10.1038/s41592-019-0654-x.
The approach used in the scripts is based on the Bioconductor e-book "Orchestrating single-cell analysis" found at http://bioconductor.org/books/release/OSCA/
Dataset used was acquired from HPAP Database: Kaestner, K. H. et al. (2019) NIH Initiative to Improve Understanding of the Pancreas, Islet, and Autoimmunity in Type 1 Diabetes:The Human Pancreas Analysis Program (HPAP), Diabetes, 68(7), pp. 1394–1402. doi: 10.2337/db19-0058