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samples.tsv

Kivanc Corut edited this page May 12, 2023 · 2 revisions

Sample Sheet: samples.tsv

Add samples information to config/samples.tsv. For each sample, you need to specify the sample name under the sample_name column and the corresponding library name under the library_name column.

  • Each sample has a library_name, which can be e.g. a library name, sequencing run or lane.
  • Each sample has a sample_name, which associates it with the biological sample it comes from.
  • For each sample, define the paths for your (columns fq1, fq2) FASTQ files (these can point to any path in your system).
  • As an alternative, you can provide an SRA (sequence read archive) accession (starting with e.g. ERR or SRR) by using a column sra. If ypu provde SRA accession instead of FASTQ file paths, the workflow will automatically download the corresponding paired end reads from SRA. If both local file paths and SRA accession are provided, the local files will be preferred.

Example samples.tsv with local files:

sample_name library_name fq1 fq2 sra
33-16 HB1JEADXX_GATCAG /path/to/your/local/fastq_1/33-16_HB1JEADXX_GATCAG_1.fastq.gz /path/to/your/local/fastq_2/33-16_HB1JEADXX_GATCAG_2.fastq.gz
33-16 HB1LCADXX_GATCAG /path/to/your/local/fastq_1/33-16_HB1LCADXX_GATCAG_1.fastq.gz /path/to/your/local/fastq_2/33-16_HB1LCADXX_GATCAG_2.fastq.gz
33-16 HLC27CCXX_L7 /path/to/your/local/fastq_1/33-16/33-16_HLC27CCXX_L7_1.fastq.gz /path/to/your/local/fastq_2/33-16_HLC27CCXX_L7_2.fastq.gz
38-11 HLCY2CCXX_L3 /path/to/your/local/fastq_1/38-11/38-11_HLCY2CCXX_L3_1.fastq.gz /path/to/your/local/fastq_2/38-11_HLCY2CCXX_L3_2.fastq.gz
4226 H174VBGXX /path/to/your/local/fastq_1/4226/4226_H174VBGXX_1.fastq.gz /path/to/your/local/fastq_2/4226_H174VBGXX_2.fastq.gz
4226 HLC27CCXX_L3 /path/to/your/local/fastq_1/4226/4226_HLC27CCXX_L3_1.fastq.gz /path/to/your/local/fastq_2/4226/4226_HLC27CCXX_L3_2.fastq.gz
4722 H3CWKBGXX_GCCAAT /path/to/your/local/fastq_1/4722_H3CWKBGXX_GCCAAT_1.fastq.gz /path/to/your/local/fastq_2/4722_H3CWKBGXX_GCCAAT_2.fastq.gz
4722 HLCY2CCXX_L3 /path/to/your/local/fastq_1/4722_HLCY2CCXX_L3_1.fastq.gz /path/to/your/local/fastq_2/4722_HLCY2CCXX_L3_2.fastq.gz
A188 HLC27CCXX_L3 /path/to/your/local/fastq_1/A188_HLC27CCXX_L3_1.fastq.gz /path/to/your/local/fastq_2/A188_HLC27CCXX_L3_2.fastq.gz
A188 H174VBGXX /path/to/your/local/fastq_1/A188_H174VBGXX_1.fastq.gz /path/to/your/local/fastq_2/A188_H174VBGXX_2.fastq.gz

Example samples.tsv with SRA accessions:

sample_name library_name fq1 fq2 sra
C00022105 C00022105 SRR3050845
C00022106 C00022106 SRR3050846
C00022107 C00022107 SRR3050847
C00022108 C00022108 SRR3050848
C00022109 C00022109 SRR3050849
C00022110 C00022110 SRR3050850
C00022119 C00022119 SRR3050851
C00022112 C00022112 SRR3050852
C00022113 C00022113 SRR3050853
C00022114 C00022114 SRR3050854