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Update README.org
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thackl committed Jan 27, 2015
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Expand Up @@ -230,8 +230,8 @@ results in repeated runs. In particular, one feature is prone to generate
differences when employed in proovread's iterative strategy: for performance
reasons bwa encodes nucleotides using 2 bits only, meaning bwa only has a
four letter alphabet =[ATGC]=. Other bases, including =NNNN= stretches used
for masking by proovread, are converted into random =[ATGC]= strings. This
has the most severe effect on alignments at the margins of masked regions:
for masking by proovread, are converted into random =[ATGC]= strings. This,
in particular, effects alignments at the margins of masked regions:
#+BEGIN_EXAMPLE
orig | ATGAATTGGTTAATCTGC
masked | ATGAATTGGTNNNNNNNN
Expand All @@ -247,15 +247,14 @@ has the most severe effect on alignments at the margins of masked regions:
#+END_EXAMPLE

***** sorting with threshold
whenever there are decisions to make based on some sort of sorted list in
combination with fixed amount of items to keep/remove, things get
non-deterministic, if different items can have identical values in their sorting
fields. In proovread, this for example affects filtering of "best alignments" in
bins (local context).
Whenever there are decisions to make for sorted list in combination with fixed
amount of items to keep/remove, things get non-deterministic if identical values in sorting
fields occur. In proovread, this for example affects filtering of "best alignments" in
bins (localized scoring context).

***** consensus calling
Whenever there is a 50-50 ratio for a base call, the resulting base is randomly
chosen, to minimize particular biases.
50-50 ratios in base calling will result in one randomly
chosen alternative, minimizing a particular bias.

** Algorithm and Implementation
Algorithm and Implementation are described in detail in the [[http://dx.doi.org/10.1093/bioinformatics/btu392][proovread]] paper.
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