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FAQ
Q1: ssGSEA missing p value and FDR?
A: The original ssGSEA alogrithm will not give you pval or FDR, so, please ignore the gseaplot generated by ssgsea
. It's useless and misleading, therefore, fdr, and pval are not shown on the plot. If you'er seeking for ssGSEA with p-value output, please see here: https://github.com/broadinstitute/ssGSEA2.0
Actually, ssGSEA2.0 use the same method with GSEApy to calculate P-value, but FDR is not.
Q2: How to save plots using gseaplot
, barplot
, dotplot
,heatmap
in Jupyter?
A: e.g. gseaplot(...ofname='your.plot.pdf'). That's it
Q3: I want to use python 2 version of GSEApy.
A: Not recommended. I'll not fix the bugs in py2 version. But if you need, install v0.9.4 and see the docs here: https://gseapy.readthedocs.io/en/v0.9.4/index.html.
Q4: What cutoff
mean in functions, like enrichr()
, dotplot
,barplot
?
A: This argument control the terms (e.g FDR < 0.05) that will be shown on figures, not the result table ouput.