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对于一个可以正常传代、正常重组的二倍体,即使杂合度很高,subphaser也不能分开。subphaser理论上只能分开异源的二倍体F1代(或F2?)。
2024-08-05 15:21:20"jenny315" ***@***.***>写道:
张老师好! 我组装了一个2倍体基因组, 因为做kmer分析的时候, 整个基因组的杂合度很高, 所以选择装出两套单倍型基因组,我想用subphaser来对这两套单倍型基因组进行分型, -f 参数默认是2,如果我使用-f 默认参数就显示ValueError: 0 kmer remained after filtering. Please reset the filter options. 后来我将-f参数设为1.6, 跑出来的结果发现染色体分配不平均, 一组为10条,一组为16条。这种情况应该怎样处理,能使得染色体平均分组?
我的代码: subphaser -i wzmt.fasta -c sp.config -disable_ltr -f 1.6 -pre sp_out > sp.log
sp_outk15_q200_f1.6.circos.pdf
sp_outk15_q200_f1.6.kmer.mat.pdf
sp_outk15_q200_f1.6.kmer_freq.pdf
sp_outk15_q200_f1.6.kmer_pca.pdf
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张老师好! 我组装了一个2倍体基因组, 因为做kmer分析的时候, 整个基因组的杂合度很高, 所以选择装出两套单倍型基因组,我想用subphaser来对这两套单倍型基因组进行分型, -f 参数默认是2,如果我使用-f 默认参数就显示ValueError: 0 kmer remained after filtering. Please reset the filter options. 后来我将-f参数设为1.6, 跑出来的结果发现染色体分配不平均, 一组为10条,一组为16条。这种情况应该怎样处理,能使得染色体平均分组?
我的代码: subphaser -i wzmt.fasta -c sp.config -disable_ltr -f 1.6 -pre sp_out > sp.log
sp_outk15_q200_f1.6.circos.pdf
sp_outk15_q200_f1.6.kmer.mat.pdf
sp_outk15_q200_f1.6.kmer_freq.pdf
sp_outk15_q200_f1.6.kmer_pca.pdf
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