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Bulk gene expression matrix format #3

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dlubawska opened this issue Aug 26, 2022 · 0 comments
Open

Bulk gene expression matrix format #3

dlubawska opened this issue Aug 26, 2022 · 0 comments

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@dlubawska
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Apologies for perhaps a silly question - an undergrad here! :)
Regarding the Y input file - should I use TPM or read count expression matrix? I've obtained both of those after pre-processing raw RNAseq reads with Trimmomatic & applying Kallisto for gene expression quantification (using the quanTIseq pipeline).

Y: a Ngene by Nsample matrix contains bulk gene expression data. This should be in linear-scale data without log-transformation.

Thank you in advance!

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