Inconsistent sequence and quality length in output fastq file

[Morpheus2112]

Dear,

When I run sortmerna on paired-end files, the output file contains reads, where the length of the sequence and relevant quality line doesn't match. Here is one example:

I am wondering is this a normal thing or else, because I have not met this issue before.

Thanks.
JZ

[biocodz]

What version of the application do you use? Could you provide the execution trace?

[Morpheus2112]

Hi, The version is 2.1b and below is the execution trace I obtained. Best, JZ biocodz ***@***.***> 于2023年11月23日周四 12:20写道：

…

What version of the application do you use? Could you provide the execution trace <https://github.com/sortmerna/sortmerna#execution-trace>? — Reply to this email directly, view it on GitHub <#407 (comment)>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/ACBBPSLDJJSS7PGNXOTUTUDYF4WQVAVCNFSM6AAAAAA7WHBBKWVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMYTQMRUGI2TGNBWGQ> . You are receiving this because you authored the thread.Message ID: ***@***.***>

-- 张继昌（Zhang Jichang） Bioinformatician IOR USI Tel:+86 13102117291 +41 766378754 Add:Room 3, Via Motto di Mornera 1, Bellinzona, CH Code:6500

[biocodz]

I would suggest to switch to the latest 4.3.6. A lot of bugs were fixed since 2.1