diff --git a/.rubocop_todo.yml b/.rubocop_todo.yml
index dc8ae12d55..32464e1c24 100644
--- a/.rubocop_todo.yml
+++ b/.rubocop_todo.yml
@@ -102,6 +102,7 @@ Lint/EmptyBlock:
     - 'app/api/endpoints/suppliers.rb'
     - 'app/api/endpoints/tag2_layout_templates.rb'
     - 'app/api/endpoints/tag_groups.rb'
+    - 'app/api/endpoints/tag_sets.rb'
     - 'app/api/endpoints/tag_layout_templates.rb'
     - 'app/api/endpoints/transfer_requests.rb'
     - 'app/api/endpoints/transfer_templates.rb'
diff --git a/app/controllers/api/v2/tag_sets_controller.rb b/app/controllers/api/v2/tag_sets_controller.rb
new file mode 100644
index 0000000000..3b707fdc42
--- /dev/null
+++ b/app/controllers/api/v2/tag_sets_controller.rb
@@ -0,0 +1,19 @@
+# frozen_string_literal: true
+
+module Api
+  module V2
+    # Provides a JSON API controller for TagSet
+    # See: http://jsonapi-resources.com/ for JSONAPI::Resource documentation
+    class TagSetsController < JSONAPI::ResourceController
+      # By default JSONAPI::ResourceController provides most the standard
+      # behaviour, and in many cases this file may be left empty.
+      before_action :check_feature_flag
+
+      private
+
+      def check_feature_flag
+        render json: { error: 'TagSets API is disabled' } unless Flipper.enabled?(:y24_220_enable_tag_set_api)
+      end
+    end
+  end
+end
diff --git a/app/models/tag_set.rb b/app/models/tag_set.rb
index 6e068d6a5c..ddd1a206a0 100644
--- a/app/models/tag_set.rb
+++ b/app/models/tag_set.rb
@@ -4,6 +4,7 @@
 # It can also be used to represent single index tag sets
 # Background explained in Y24-170 (https://github.com/sanger/sequencescape/issues/4160)
 class TagSet < ApplicationRecord
+  include Uuid::Uuidable
   # For dual index tags, tag_group is i7 oligos and tag2_group is i5 oligos
   belongs_to :tag_group, class_name: 'TagGroup', optional: false
 
@@ -36,6 +37,7 @@ class TagSet < ApplicationRecord
   # The scoping retrieves the visible tag sets and makes sure they are dual index.
   scope :visible_dual_index, -> { dual_index.visible }
 
+  scope :by_adapter_type, ->(adapter_type_name) { joins(:tag_group).merge(TagGroup.by_adapter_type(adapter_type_name)) }
   scope :single_index, -> { where(tag2_group: nil) }
 
   scope :visible_single_index, -> { single_index.visible }
diff --git a/app/resources/api/v2/tag_set_resource.rb b/app/resources/api/v2/tag_set_resource.rb
new file mode 100644
index 0000000000..00cae0b7c0
--- /dev/null
+++ b/app/resources/api/v2/tag_set_resource.rb
@@ -0,0 +1,77 @@
+# frozen_string_literal: true
+
+module Api
+  module V2
+    # Provides a JSON:API representation of {TagSet}, which links together two related tag groups.
+    # A TagSet represents a logical grouping of tags used for indexing in sequencing experiments.
+    # It typically consists of a primary tag group and an optional secondary tag group,
+    # enabling support for both single and dual indexing workflows.
+    # This resource allows clients to query, filter, and retrieve information about tag sets,
+    # including their associated tag groups and metadata, through the `/api/v2/tag_sets/` endpoint.
+    #
+    # @note Access this resource via the `/api/v2/tag_sets/` endpoint.
+    #
+    # @example GET request for all TagSet resources
+    #   GET /api/v2/tag_sets/
+    #
+    # @example GET request for a specific TagSet by ID
+    #   GET /api/v2/tag_sets/123/
+    #
+    # @example Filtering by tag group adapter type name
+    #   GET /api/v2/tag_sets/?filter[tag_group_adapter_type_name]=AdapterType1
+    #
+    # @example Filtering by visibility (applied by default)
+    #   GET /api/v2/tag_sets/?filter[visible]=true
+    #
+    # For more information about JSON:API, see the [JSON:API Specifications](https://jsonapi.org/format/)
+    # or the [JSONAPI::Resources](http://jsonapi-resources.com/) package, which implements JSON:API for Sequencescape.
+    class TagSetResource < BaseResource
+      immutable
+
+      default_includes :uuid_object, :tag_group, :tag2_group
+
+      ###
+      # Relationships
+      ###
+
+      # @!attribute [r] tag_group
+      #   A relationship for the primary tag group associated with the tag layout template.
+      #   @return [Api::V2::TagGroupResource]
+      has_one :tag_group, readonly: true
+
+      # @!attribute [r] tag2_group
+      #   A relationship for the secondary tag group associated with the tag layout template.
+      #   This is used during dual indexing, but will not be found during single indexing.
+      #   @return [Api::V2::TagGroupResource]
+      has_one :tag2_group, readonly: true
+
+      ###
+      # Attributes
+      ###
+
+      # @!attribute [r] uuid
+      #   The UUID of the tag set.
+      #   @return [String].
+      attribute :uuid, readonly: true
+
+      # @!attribute [r] name
+      #   The display name of the tag set.
+      #   @return [String]
+      attribute :name, readonly: true
+
+      ###
+      # Filters
+      ###
+
+      # Allows filtering by the adapter type name of the tag group.
+      # @example
+      #   GET /api/v2/tag_sets/?filter[tag_group_adapter_type_name]=AdapterType1
+      filter :tag_group_adapter_type_name, apply: ->(records, value, _options) { records.by_adapter_type(value) }
+
+      # Allows filtering by visibility.
+      # @example
+      #   GET /api/v2/tag_sets/?filter[visible]=true
+      filter :visible, default: true, apply: ->(records, _value, _options) { records.visible }
+    end
+  end
+end
diff --git a/config/feature_flags.yml b/config/feature_flags.yml
index 719af38eed..76f57412a0 100644
--- a/config/feature_flags.yml
+++ b/config/feature_flags.yml
@@ -4,3 +4,5 @@
 dpl_395_2_enable_advanced_search_tab: Shows the Search tab in the top navigation bar
 # Below is flagged off until some existing data that would violate the rule is fixed. Should be re-enabled in Y24-058.
 y24_052_enable_data_release_timing_validation: Enables server-side validation that enforces a relationship between the values of two study metadata fields
+# Below is disabled until the new API is ready for production. This is dependent on Y24-185, which involves creating tag sets for all tag groups used for CustomTaggedPlates.
+y24_220_enable_tag_set_api: Enables the Tag Set API
diff --git a/config/routes.rb b/config/routes.rb
index 544494cc5a..896dd8d524 100644
--- a/config/routes.rb
+++ b/config/routes.rb
@@ -67,6 +67,7 @@
       jsonapi_resources :submissions
       jsonapi_resources :tag_group_adapter_types
       jsonapi_resources :tag_groups
+      jsonapi_resources :tag_sets, only: %i[index show]
       jsonapi_resources :tag_layout_templates
       jsonapi_resources :tag_layouts, except: %i[update]
       jsonapi_resources :tags
diff --git a/spec/requests/api/v2/labware_spec.rb b/spec/requests/api/v2/labware_spec.rb
index f648eebba7..a27b80d45a 100644
--- a/spec/requests/api/v2/labware_spec.rb
+++ b/spec/requests/api/v2/labware_spec.rb
@@ -12,22 +12,15 @@
     before do
       create(:sample_tube)
       create(:library_tube)
-    end
-
-    it 'sends a list of labware' do
       api_get base_endpoint
+    end
 
-      # test for the 200 status-code
+    it 'responds with a success http code when retrieving a list of receptacles.' do
       expect(response).to have_http_status(:success)
-
-      # check to make sure the right amount of messages are returned
-      expect(json['data'].length).to eq(2)
     end
 
-    it 'identifies the type of labware' do
-      api_get base_endpoint
-      listed = json['data'].pluck('type').sort
-      expect(listed).to eq(%w[tubes tubes])
+    it 'returns the correct number of receptacles' do
+      expect(json['data'].length).to eq(2)
     end
 
     # Check filters, ESPECIALLY if they aren't simple attribute filters
diff --git a/spec/requests/api/v2/tag_sets_spec.rb b/spec/requests/api/v2/tag_sets_spec.rb
new file mode 100644
index 0000000000..3c008e8509
--- /dev/null
+++ b/spec/requests/api/v2/tag_sets_spec.rb
@@ -0,0 +1,145 @@
+# frozen_string_literal: true
+
+require 'rails_helper'
+require './spec/requests/api/v2/shared_examples/api_key_authenticatable'
+require './spec/requests/api/v2/shared_examples/requests'
+
+describe 'TagSets API', with: :api_v2 do
+  let(:model_class) { TagSet }
+  let(:base_endpoint) { '/api/v2/tag_sets' }
+  let(:resource_type) { model_class.name.demodulize.pluralize.underscore }
+
+  before { Flipper.enable(:y24_220_enable_tag_set_api) }
+
+  it_behaves_like 'ApiKeyAuthenticatable'
+
+  context 'with multiple TagSets' do
+    before do
+      create_list(:tag_set, 5)
+      create(:tag_set, tag_group: create(:tag_group, visible: false), tag2_group: create(:tag_group))
+      api_get base_endpoint
+    end
+
+    it 'responds with a success http code when sending a list of tag_sets with visible tag groups' do
+      expect(response).to have_http_status(:success)
+    end
+
+    it 'returns the correct number of tag_sets with visible tag groups' do
+      expect(json['data'].length).to eq(5)
+    end
+  end
+
+  context 'when filtering tag sets by tag_group_adapter_type_name' do
+    before do
+      adapter_type = build(:adapter_type, name: 'adapter_type_1')
+      create(:tag_set, tag_group: create(:tag_group, adapter_type:), tag2_group: create(:tag_group, adapter_type:))
+      create_list(:tag_set, 5)
+      api_get "#{base_endpoint}?filter[tag_group_adapter_type_name]=adapter_type_1"
+    end
+
+    it 'responds with a success http code when filtering by tag_group_adapter_type_name' do
+      expect(response).to have_http_status(:success)
+    end
+
+    it 'returns the correct number of tag_sets when filtering by tag_group_adapter_type_name' do
+      expect(json['data'].length).to eq(1)
+    end
+
+    it 'returns the correct tag_set when filtering by tag_group_adapter_type_name' do
+      expect(json['data'][0]['attributes']['name']).to eq(TagSet.first.name)
+    end
+  end
+
+  context 'with a single resource' do
+    let(:resource) { create(:tag_set) }
+
+    context 'without included relationships' do
+      before { api_get "#{base_endpoint}/#{resource.id}" }
+
+      it 'responds with a success http code' do
+        expect(response).to have_http_status(:success)
+      end
+
+      it 'returns the correct resource id' do
+        expect(json.dig('data', 'id')).to eq(resource.id.to_s)
+      end
+
+      it 'returns the correct resource type' do
+        expect(json.dig('data', 'type')).to eq(resource_type)
+      end
+
+      it 'returns the correct resource name' do
+        expect(json.dig('data', 'attributes', 'name')).to eq(resource.name)
+      end
+
+      it 'returns the correct resource uuid' do
+        expect(json.dig('data', 'attributes', 'uuid')).to eq(resource.uuid)
+      end
+
+      it 'returns reference to tag_group' do
+        expect(json.dig('data', 'relationships', 'tag_group')).to be_present
+      end
+
+      it 'returns reference to tag2_group' do
+        expect(json.dig('data', 'relationships', 'tag2_group')).to be_present
+      end
+    end
+  end
+
+  context 'with included relationships' do
+    let(:resource) { create(:tag_set) }
+
+    it_behaves_like 'a GET request including a has_one relationship', 'tag_group'
+    it_behaves_like 'a GET request including a has_one relationship', 'tag2_group'
+  end
+
+  context 'with included tags for tag groups' do
+    let(:resource) { create(:tag_set, tag_group:, tag2_group:) }
+    let(:tag_group) { create(:tag_group, tags:) }
+    let(:tag2_group) { create(:tag_group, tags: tags2) }
+    let(:tags) do
+      [
+        build(:tag, oligo: 'AAA', map_id: 1),
+        build(:tag, oligo: 'TTT', map_id: 2),
+        build(:tag, oligo: 'CCC', map_id: 3),
+        build(:tag, oligo: 'GGG', map_id: 4)
+      ]
+    end
+    let(:tags2) do
+      [
+        build(:tag, oligo: 'TTT', map_id: 1),
+        build(:tag, oligo: 'AAA', map_id: 2),
+        build(:tag, oligo: 'CCC', map_id: 3),
+        build(:tag, oligo: 'GGG', map_id: 4)
+      ]
+    end
+
+    before { api_get "#{base_endpoint}/#{resource.id}?include=tag_group,tag2_group" }
+
+    it 'returns a success http code' do
+      expect(response).to have_http_status(:success)
+    end
+
+    it 'returns tags for the first tag group within the tag set in included' do
+      expect(json['included'][0]['attributes']['tags']).to eq(
+        [
+          { 'index' => 1, 'oligo' => 'AAA' },
+          { 'index' => 2, 'oligo' => 'TTT' },
+          { 'index' => 3, 'oligo' => 'CCC' },
+          { 'index' => 4, 'oligo' => 'GGG' }
+        ]
+      )
+    end
+
+    it 'returns tags for the second tag group within the tag set in included' do
+      expect(json['included'][1]['attributes']['tags']).to eq(
+        [
+          { 'index' => 1, 'oligo' => 'TTT' },
+          { 'index' => 2, 'oligo' => 'AAA' },
+          { 'index' => 3, 'oligo' => 'CCC' },
+          { 'index' => 4, 'oligo' => 'GGG' }
+        ]
+      )
+    end
+  end
+end
diff --git a/spec/resources/api/v2/tag_set_resource_spec.rb b/spec/resources/api/v2/tag_set_resource_spec.rb
new file mode 100644
index 0000000000..17bf118302
--- /dev/null
+++ b/spec/resources/api/v2/tag_set_resource_spec.rb
@@ -0,0 +1,37 @@
+# frozen_string_literal: true
+
+require 'rails_helper'
+require './app/resources/api/v2/tag_group_resource'
+
+RSpec.describe Api::V2::TagSetResource, type: :resource do
+  subject(:resource) { described_class.new(resource_model, {}) }
+
+  let(:resource_model) { build_stubbed(:tag_set, tag_group:, tag2_group:) }
+  let(:tag_group) { create(:tag_group) }
+  let(:tag2_group) { create(:tag_group) }
+
+  it { is_expected.to have_model_name 'TagSet' }
+
+  # Test attributes
+  it 'exposes attributes', :aggregate_failures do
+    expect(resource).to have_attribute :uuid
+    expect(resource).to have_attribute :name
+    expect(resource).not_to have_updatable_field(:id)
+    expect(resource).not_to have_updatable_field(:uuid)
+    expect(resource).not_to have_updatable_field(:name)
+  end
+
+  # Relationships
+  it { is_expected.to have_a_readonly_has_one(:tag_group).with_class_name('TagGroup') }
+  it { is_expected.to have_a_readonly_has_one(:tag2_group).with_class_name('TagGroup') }
+
+  describe '#tag group' do
+    it 'returns the correct tag group information' do
+      expect(resource.tag_group._model).to eq(tag_group)
+    end
+
+    it 'returns the correct tag2 group information' do
+      expect(resource.tag2_group._model).to eq(tag2_group)
+    end
+  end
+end