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Hello, I'm trying to troubleshoot why my mitochondrial contig wasn't circularized, and in 03.assemble>warnings.log I found
* 0:00:03.219 15M / 74M WARN General (launcher.cpp : 172) Your data seems to have high uniform coverage depth. It is strongly recommended to use --isolate option.
Therefore I tried adding --assemble_spades_isolate, but get circlator: error: unrecognized arguments: --assemble_spades_isolate.
Is there a different way the isolate option should be added?
Best regards,
Brett
The text was updated successfully, but these errors were encountered:
brettmorg
changed the title
Using spades --isolate option in circulator all
Using spades --isolate option in circlator all
Nov 2, 2020
Hello, I'm trying to troubleshoot why my mitochondrial contig wasn't circularized, and in 03.assemble>warnings.log I found
* 0:00:03.219 15M / 74M WARN General (launcher.cpp : 172) Your data seems to have high uniform coverage depth. It is strongly recommended to use --isolate option.
Therefore I tried adding
--assemble_spades_isolate
, but getcirclator: error: unrecognized arguments: --assemble_spades_isolate
.Is there a different way the isolate option should be added?
Best regards,
Brett
The text was updated successfully, but these errors were encountered: