(API Page) Present in constructors of FluentLocalization and FluentBundle, the use_isolating parameter is not explained. It does not seem to be used either: FluentLocalization delegates it to FluentBundle, and FluentBundle doesn't use it for anything (except that FluentBundle passes self to ResolverEnvironment, which may expose self.use_isolating, but I did not check further).
In addition, the default value of use_isolating in FluentLocalization constructor is False, while in FluentBundle's it is True.