From 9e74324cc0b52700f84d738a74e6248a1b634665 Mon Sep 17 00:00:00 2001 From: Gregory Jefferis Date: Mon, 22 Jul 2019 18:25:35 +0100 Subject: [PATCH] redown --- docs/articles/Installation.html | 18 +- docs/articles/NeuroGeometry.html | 316 +++++++++++------ docs/articles/index.html | 2 +- docs/articles/neurons-as-graph.html | 132 +++---- .../figure-html/unnamed-chunk-5-1.png | Bin 111640 -> 106108 bytes docs/articles/neurons-intro.html | 325 ++++++++++++------ .../figure-html/unnamed-chunk-34-1.png | Bin 34128 -> 48372 bytes .../figure-html/unnamed-chunk-34-2.png | Bin 37793 -> 51683 bytes docs/authors.html | 4 +- docs/index.html | 51 +-- docs/news/index.html | 31 +- docs/pkgdown.yml | 2 +- docs/reference/Cell07PNs.html | 2 +- docs/reference/MBL.surf.html | 2 +- docs/reference/affmat2cmtkparams.html | 2 +- docs/reference/all.equal.dotprops.html | 2 +- docs/reference/all.equal.im3d.html | 2 +- docs/reference/all.equal.neuron.html | 2 +- docs/reference/amiramesh-io.html | 2 +- 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b/docs/articles/Installation.html index 1d21c7db..1d15e21b 100644 --- a/docs/articles/Installation.html +++ b/docs/articles/Installation.html @@ -30,7 +30,7 @@ nat - 1.9.0 + 1.9.1 @@ -98,7 +98,7 @@

Installation details

Gregory Jefferis

-

2019-01-10

+

2019-07-22

Source: vignettes/Installation.Rmd @@ -127,20 +127,20 @@

Basic Installation

As of v1.0 there is a released version on CRAN. This is normally updated only every few months.

-
install.packages("nat")
+
install.packages("nat")

If you wish to run the package tests, it is necessary to install with all dependencies:

-
install.packages("nat", dependencies=TRUE)
+
install.packages("nat", dependencies=TRUE)

Development version

nat remains under quite active development, so we generally suggest using the development version directly from github. The recommended way to do this is to install Hadley Wickham’s invaluable devtools package (if you have not already done so) and then use that to install nat.

-
# install devtools if required
-if (!require("devtools")) install.packages("devtools")
-# then install nat
-devtools::install_github("jefferis/nat")
+

The nat package includes extensive unit tests which are run along with R’s (extremely fastidious) package check routines by the Travis continuous integration server. The master branch is therefore considered very stable and may well contain fixes or enhancements over released versions. However, you can install any release version including the latest release as follows:

-
devtools::install_github("jefferis/nat@v1.8.6")
+
devtools::install_github("jefferis/nat@v1.8.6")

The same syntax can be used to install any arbitrary version that you want from github. See ?install_github for details.

Note: Windows users need Rtools to install in this way, but devtools should offer to install this for you if you do not already have it.

diff --git a/docs/articles/NeuroGeometry.html b/docs/articles/NeuroGeometry.html index 847f9dd1..ce183941 100644 --- a/docs/articles/NeuroGeometry.html +++ b/docs/articles/NeuroGeometry.html @@ -30,7 +30,7 @@ nat - 1.9.0 + 1.9.1 @@ -98,7 +98,7 @@

NeuroGeometry: Analysing 3D morphology of neurons

Gregory Jefferis

-

2019-01-10

+

2019-07-22

Source: vignettes/NeuroGeometry.Rmd @@ -117,17 +117,20 @@

Vertex information

-
library(nat)
+
library(nat)
## Loading required package: rgl
+
## Registered S3 method overwritten by 'nat':
+##   method             from
+##   as.mesh3d.ashape3d rgl
## 
 ## Attaching package: 'nat'
## The following objects are masked from 'package:base':
 ## 
 ##     intersect, setdiff, union

One of the key tools for geometric work in nat is the xyzmatrix function, which extracts 3-D coordinate information from any object containing vertices.

-
n=Cell07PNs[[1]]
-xyz=xyzmatrix(n)
-summary(xyz)
+
##        X               Y                Z        
 ##  Min.   :186.9   Min.   : 90.36   Min.   : 88.2  
 ##  1st Qu.:225.6   1st Qu.: 97.56   1st Qu.:103.4  
@@ -135,13 +138,13 @@ 

## Mean :249.4 Mean :104.03 Mean :120.9 ## 3rd Qu.:277.7 3rd Qu.:109.04 3rd Qu.:139.0 ## Max. :289.5 Max. :132.71 Max. :157.3

-
plot(xyz[,c("X","Y")], type='p')
+
plot(xyz[,c("X","Y")], type='p')

This also works for neuronlists, so we can extract all of the points in a collection of neurons:

-
xyz=xyzmatrix(Cell07PNs)
+
xyz=xyzmatrix(Cell07PNs)

We could ask for all the points close to a specific X coordinate

-
close_to_x250=abs(xyz[,'X']-250)<10
-table(close_to_x250)
+
close_to_x250=abs(xyz[,'X']-250)<10
+table(close_to_x250)
## close_to_x250
 ## FALSE  TRUE 
 ## 20479  1728
@@ -151,120 +154,120 @@

Worked example - plane cutting a tract

As a worked example let’s try to find the plane cutting a tract defined by the axons of some example neurons:

-
plot(Cell07PNs, WithNodes=F)
-dist=10
-abline(v=250, col='red')
-abline(v=c(250-dist, 250+dist), col='red', lty='dotted')
+
plot(Cell07PNs, WithNodes=F)
+dist=10
+abline(v=250, col='red')
+abline(v=c(250-dist, 250+dist), col='red', lty='dotted')

We could ask for all the points close to a specific X coordinate

-
close_to_x250=abs(xyz[,'X']-250)<10
-table(close_to_x250)
+
close_to_x250=abs(xyz[,'X']-250)<10
+table(close_to_x250)
## close_to_x250
 ## FALSE  TRUE 
 ## 20479  1728

Let’s find the centroid of those selected points:

-
centroid=colMeans(xyz[close_to_x250,])
-centroid
+
centroid=colMeans(xyz[close_to_x250,])
+centroid
##         X         Y         Z 
 ## 251.16788  94.76735 141.11495
-
plot(Cell07PNs, WithNodes=F)
-points(matrix(centroid[1:2],ncol=2), pch=20)
+
plot(Cell07PNs, WithNodes=F)
+points(matrix(centroid[1:2],ncol=2), pch=20)

PCA to find tract vector

Now let’s do a principal components analysis on those points

-
pc=prcomp(xyz[close_to_x250,])
-pc1=pc$rotation[,'PC1']
-plot(Cell07PNs, WithNodes=F)
-points(matrix(centroid[1:2],ncol=2), pch=20)
-res=rbind(centroid-pc1*10, centroid, centroid+pc1*10)
-lines(res[,1:2])
+
pc=prcomp(xyz[close_to_x250,])
+pc1=pc$rotation[,'PC1']
+plot(Cell07PNs, WithNodes=F)
+points(matrix(centroid[1:2],ncol=2), pch=20)
+res=rbind(centroid-pc1*10, centroid, centroid+pc1*10)
+lines(res[,1:2])

OK, perhaps it would be better if we only used the spine of the neurons

-
# There is a problem with one neuron with a loop, hence OmitFailures
-spines=nlapply(Cell07PNs, spine, UseStartPoint=T, OmitFailures = T)
+
# There is a problem with one neuron with a loop, hence OmitFailures
+spines=nlapply(Cell07PNs, spine, UseStartPoint=T, OmitFailures = T)
## Warning in get.shortest.paths(ng, from = from, to = to, mode = "all"): At
 ## structural_properties.c:4597 :Couldn't reach some vertices
-
xyz=xyzmatrix(spines)
-close_to_x250=abs(xyz[,'X']-250)<10
-table(close_to_x250)
+
xyz=xyzmatrix(spines)
+close_to_x250=abs(xyz[,'X']-250)<10
+table(close_to_x250)
## close_to_x250
 ## FALSE  TRUE 
 ##  5137   876
-
pc=prcomp(xyz[close_to_x250,])
-pc1=pc$rotation[,'PC1']
-plot(spines, WithNodes=F)
-points(matrix(centroid[1:2],ncol=2), pch=20)
-res=rbind(centroid-pc1*10, centroid, centroid+pc1*10)
-# nb 1:2 => just the xy coords
-lines(res[,1:2])
+
pc=prcomp(xyz[close_to_x250,])
+pc1=pc$rotation[,'PC1']
+plot(spines, WithNodes=F)
+points(matrix(centroid[1:2],ncol=2), pch=20)
+res=rbind(centroid-pc1*10, centroid, centroid+pc1*10)
+# nb 1:2 => just the xy coords
+lines(res[,1:2])

Tract vector for each neuron

Alternatively, we could find the vector of the first PC for each neuron. Let’s write a function to do that and then apply it to the spine of each neuron.

-
# returns 6 vector
-# first 3 values are centroid
-# second three values first principal component
-tract_vector <- function(n, xval=250, thresh=10) {
-  p=xyzmatrix(n)
-  near=abs(p[,'X']-xval)<thresh
-  pc=prcomp(p[near,])
-  c(colMeans(p[near,]), pc$rotation[,'PC1'])
-}
-tvs=sapply(spines, tract_vector)
-mean_cent=rowMeans(tvs[1:3,])
-mean_vec=rowMeans(tvs[4:6,])
-
-# nb *10 to make the vector longer (10 µm) and more visible
-res2=rbind(mean_cent-mean_vec*10, mean_cent, mean_cent+mean_vec*10)
-plot(spines, col='grey', WithNodes=F)
-lines(res2[,1:2], lwd=3)
+
# returns 6 vector
+# first 3 values are centroid
+# second three values first principal component
+tract_vector <- function(n, xval=250, thresh=10) {
+  p=xyzmatrix(n)
+  near=abs(p[,'X']-xval)<thresh
+  pc=prcomp(p[near,])
+  c(colMeans(p[near,]), pc$rotation[,'PC1'])
+}
+tvs=sapply(spines, tract_vector)
+mean_cent=rowMeans(tvs[1:3,])
+mean_vec=rowMeans(tvs[4:6,])
+
+# nb *10 to make the vector longer (10 µm) and more visible
+res2=rbind(mean_cent-mean_vec*10, mean_cent, mean_cent+mean_vec*10)
+plot(spines, col='grey', WithNodes=F)
+lines(res2[,1:2], lwd=3)

Lines crossing a plane

We can look at the plane we have defined in a 3D context as follows:

-
plc=plane_coefficients(mean_cent, mean_vec)
-plot3d(Cell07PNs[c(1,30)], col='grey', WithNodes = F)
-planes3d(plc[,1:3], d=plc[,'d'])
+
plc=plane_coefficients(mean_cent, mean_vec)
+plot3d(Cell07PNs[c(1,30)], col='grey', WithNodes = F)
+planes3d(plc[,1:3], d=plc[,'d'])

We can also make a small square plane centred on the chosen point

-
library(rgl)
-clear3d()
-make_centred_plane <- function(point, normal, scale = 1) {
-  # find the two orthogonal vectors to this one
-  uv = Morpho::tangentPlane(normal)
-  uv = sapply(uv, "*", scale, simplify = F)
-  qmesh3d(
-  cbind(
-  point + uv$z,
-  point + uv$y,
-  point - uv$z,
-  point - uv$y
-  ),
-  homogeneous = F,
-  indices = 1:4
-  )
-}
-plane=make_centred_plane(mean_cent, mean_vec, scale=15)
-plot3d(spines, col='grey')
-shade3d(plane, col='red')
+

Finally, we can find the positions at which each neuron intersects the plane. Note that when there are multiple intersections, we only choose the closest point to the centroid that we calculated when defining the plane.

-
intersections=t(sapply(Cell07PNs, intersect_plane, plc, closestpoint=mean_cent))
-# center those intersection points
-intersections.cent=scale(intersections, center = T, scale = F)
-d=sqrt(rowSums(intersections.cent^2))
-mean(d)
+
intersections=t(sapply(Cell07PNs, intersect_plane, plc, closestpoint=mean_cent))
+# center those intersection points
+intersections.cent=scale(intersections, center = T, scale = F)
+d=sqrt(rowSums(intersections.cent^2))
+mean(d)
## [1] 3.118692

Then we can plot those positions on the plane

-
spheres3d(intersections, col='red', rad=.5)
-shade3d(plane, col='black')
-plot3d(spines, col='grey')
+
spheres3d(intersections, col='red', rad=.5)
+shade3d(plane, col='black')
+plot3d(spines, col='grey')

You must enable Javascript to view this page properly.

Finally we can construct a 2D coordinate system on the plane and project the intersection positions onto that.

-
# find pair of orthogonal tangent vectors of plane
-uv = Morpho::tangentPlane(mean_vec)
-# centre our points on mean
-sp=scale(intersections, scale = F, center=T)
-DotProduct=function(a,b) sum(a*b)
-# find coordinates w.r.t. to our two basis vectors
-xy=data.frame(u=apply(sp, 1, DotProduct, uv[[1]]),
-           v=apply(sp, 1, DotProduct, uv[[2]]))
-# maintain consist x-y aspect ratio 
-plot(xy, pch=19, asp=1, main = "Position of Axon intersection in plane", 
-     xlab="u /µm", ylab="v /µm")
+
# find pair of orthogonal tangent vectors of plane
+uv = Morpho::tangentPlane(mean_vec)
+# centre our points on mean
+sp=scale(intersections, scale = F, center=T)
+DotProduct=function(a,b) sum(a*b)
+# find coordinates w.r.t. to our two basis vectors
+xy=data.frame(u=apply(sp, 1, DotProduct, uv[[1]]),
+           v=apply(sp, 1, DotProduct, uv[[2]]))
+# maintain consist x-y aspect ratio 
+plot(xy, pch=19, asp=1, main = "Position of Axon intersection in plane", 
+     xlab="u /µm", ylab="v /µm")

diff --git a/docs/articles/index.html b/docs/articles/index.html index 0f96968f..8b23b987 100644 --- a/docs/articles/index.html +++ b/docs/articles/index.html @@ -60,7 +60,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/articles/neurons-as-graph.html b/docs/articles/neurons-as-graph.html index 538cff6f..ee1531f8 100644 --- a/docs/articles/neurons-as-graph.html +++ b/docs/articles/neurons-as-graph.html @@ -30,7 +30,7 @@ nat - 1.9.0 + 1.9.1 @@ -98,7 +98,7 @@

Working with individual neurons as graph structures

Gregory Jefferis

-

2019-01-10

+

2019-07-22

Source: vignettes/neurons-as-graph.Rmd @@ -135,17 +135,17 @@

  • are not in the same order as the vertices
  • have some other significance e.g. are globally unique across all neurons in a database and therefore have large values (which may be challenging for R to to represent give its maxint of 2^31 - 1=2.147483610^{9})
  • -
    library(nat)
    -n=Cell07PNs[[1]]
    -summary(n)
    +
    ##   root nodes segments branchpoints endpoints cable.length
     ## 1    1   180       33           16        18     297.1763

    We can extract the points as follows:

    - +
    ## [1] 1
    - +
    ##  [1]  34  48  51  75  78  95  98  99 108 109 115 119 135 143 160 169
    - +
    ##  [1]   1  42  59  62  80  85  96 100 102 112 117 121 134 148 154 165 172
     ## [18] 180

    Segments are unbranched connected sequences of nodes that terminate in a branch point or end point.

    @@ -158,22 +158,22 @@

    Strahler Order

    The branching structure of a neuron is commonly summarised by calculating the Strahler Order.

    -
    n=Cell07PNs[[1]]
    -so=strahler_order(n)
    -orders=1:max(so$points)
    -for (i in orders) {
    -  plot(subset(n, so$points==i), col=i, add = i!=1, boundingbox = boundingbox(n))
    -}
    -

    Note the use of multiple calls to plot.neuron using the add=FALSE argument for all but the first plot. Note also the use of the boundingbox argument/function in order to ensure that the plot is set up with appropriate axes for the whole neuron even if only part of it is plotted in the first call to plot.

    +
    n=Cell07PNs[[1]]
    +so=strahler_order(n)
    +orders=1:max(so$points)
    +for (i in orders) {
    +  plot(subset(n, so$points==i), col=i, add = i!=1, boundingbox = boundingbox(n))
    +}
    +

    Note the use of multiple calls to plot.neuron using the add=TRUE argument for all but the first plot. Note also the use of the boundingbox argument/function in order to ensure that the plot is set up with appropriate axes for the whole neuron even if only part of it is plotted in the first call to plot.

    Spine

    You can find the longest path across a neuron using the spine function.

    -
    n=Cell07PNs[[1]]
    -sp=spine(n)
    -plot(n, col='grey')
    -plot(sp, add=T, col='blue')
    +
    n=Cell07PNs[[1]]
    +sp=spine(n)
    +plot(n, col='grey')
    +plot(sp, add=T, col='blue')

    spine has a variety of options that you can use to control the results.

    @@ -181,8 +181,8 @@

    Segment graph

    You can use the segmentgraph function to make a simplified representation of the branching structure of the neuron. In this object (which has class igraph) each unbranched segment in the original neuron (which might have contained many vertices) is collapsed to a single edge joining the branch points (which are retained).

    -
    sg=segmentgraph(Cell07PNs[[1]])
    -plot(sg)
    +
    sg=segmentgraph(Cell07PNs[[1]])
    +plot(sg)

    @@ -190,13 +190,13 @@

    ngraph objects

    The nat package provides a bridge to a rich cross-platform graph library, igraph. We provide a class ngraph that provides a thin wrapper for the igraph class. This looks after things that we might need to know about a neuron (like the 3D coordinates of each node) while still giving access to all of the graph functions in the igraph package.

    -
    g=as.ngraph(Cell07PNs[[1]])
    -class(g)
    +
    g=as.ngraph(Cell07PNs[[1]])
    +class(g)
    ## [1] "ngraph" "igraph"
    -
    g
    -
    ## IGRAPH 5fdf829 DN-- 180 179 -- 
    +
    +
    ## IGRAPH b52ae67 DN-- 180 179 -- 
     ## + attr: name (v/n), X (v/n), Y (v/n), Z (v/n), diam (v/n)
    -## + edges from 5fdf829 (vertex names):
    +## + edges from b52ae67 (vertex names):
     ##  [1]  1-> 2  2-> 3  3-> 4  4-> 5  5-> 6  6-> 7  7-> 8  8-> 9  9->10 10->11
     ## [11] 11->12 12->13 13->14 14->15 15->16 16->17 17->18 18->19 19->20 20->21
     ## [21] 21->22 22->23 23->24 24->25 25->26 26->27 27->28 28->29 29->30 30->31
    @@ -207,63 +207,63 @@ 

    ## [71] 71->72 72->73 73->74 74->75 75->76 76->77 77->78 78->79 79->80 78->81 ## + ... omitted several edges

    You can use functions such as

    -
    igraph::diameter(g)
    +
    ## [1] 89

    to find the length of the longest path across the neuron. This is defined in terms of the number of intervening nodes. You can also make a graph in which the edge weights are the euclidean distance between the connected 3D nodes:

    -
    gw=as.ngraph(Cell07PNs[[1]], weights=TRUE)
    -igraph::diameter(gw)
    +
    gw=as.ngraph(Cell07PNs[[1]], weights=TRUE)
    +igraph::diameter(gw)
    ## [1] 186.0859

    This gives you the longest path length (geodesic) across the graph in units of µm in this case.

    -

    Note that although you can do library(ngraph), it adds a lot of functions to the search path, some of which have name clashes, so I often just use the package name (igraph::) prepended to the function that I want to call.

    +

    Note that although you can do library(igraph), it adds a lot of functions to the search path, some of which have name clashes, so I often just use the package name (igraph::) prepended to the function that I want to call.

    walking along ngraph objects

    You can use the graph representation of neurons e.g. to find the path between nodes.

    -
    g=as.ngraph(Cell07PNs[[1]], weights=TRUE)
    -eg=endpoints(g)
    -
    -p=igraph::shortest_paths(g, from=1, to=180)
    -p$vpath[[1]]
    -
    ## + 90/180 vertices, named, from 6ef429c:
    +
    g=as.ngraph(Cell07PNs[[1]], weights=TRUE)
    +eg=endpoints(g)
    +
    +p=igraph::shortest_paths(g, from=1, to=180)
    +p$vpath[[1]]
    +
    ## + 90/180 vertices, named, from 28ff74c:
     ##  [1] 1   2   3   4   5   6   7   8   9   10  11  12  13  14  15  16  17 
     ## [18] 18  19  20  21  22  23  24  25  26  27  28  29  30  31  32  33  34 
     ## [35] 43  44  45  46  47  48  63  64  65  66  67  68  69  70  71  72  73 
     ## [52] 74  75  86  87  88  89  90  91  92  93  94  95  97  98  103 104 105
     ## [69] 106 107 108 135 155 156 157 158 159 160 166 167 168 169 173 174 175
     ## [86] 176 177 178 179 180
    -
    # fails
    -p2=igraph::shortest_paths(g, from=180, to=1)
    +
    ## Warning in igraph::shortest_paths(g, from = 180, to = 1): At
     ## structural_properties.c:4597 :Couldn't reach some vertices
    -
    p2$vpath[[1]]
    -
    ## + 1/180 vertex, named, from 6ef429c:
    +
    +
    ## + 1/180 vertex, named, from 28ff74c:
     ## [1] 1
    -
    # mode all will find the path irrespective of direction of links, which are
    -# directed from the soma
    -p3=igraph::shortest_paths(g, from=180, to=1, mode = 'all')
    -p3$vpath[[1]]
    -
    ## + 90/180 vertices, named, from 6ef429c:
    +
    +
    ## + 90/180 vertices, named, from 28ff74c:
     ##  [1] 180 179 178 177 176 175 174 173 169 168 167 166 160 159 158 157 156
     ## [18] 155 135 108 107 106 105 104 103 98  97  95  94  93  92  91  90  89 
     ## [35] 88  87  86  75  74  73  72  71  70  69  68  67  66  65  64  63  48 
     ## [52] 47  46  45  44  43  34  33  32  31  30  29  28  27  26  25  24  23 
     ## [69] 22  21  20  19  18  17  16  15  14  13  12  11  10  9   8   7   6  
     ## [86] 5   4   3   2   1
    -
    all.equal(p$vpath[[1]], rev(p3$vpath[[1]]))
    +
    all.equal(p$vpath[[1]], rev(p3$vpath[[1]]))
    ## [1] TRUE
    -
    # just the distances - by default uses mode=all
    -igraph::distances(g, v=1, to=180)
    +
    ##        180
     ## 1 186.0859
    -
    igraph::distances(g, v=180, to=1)
    +
    igraph::distances(g, v=180, to=1)
    ##            1
     ## 180 186.0859

    You can also ask for nodes upstream or downstream of a given starting node. For example the neurons in the Cell07PNs set have a tag called AxonLHEP that defines the entry point of the axon into the lateral horn neuropil of the fly brain. Here we defined

    -
    n=Cell07PNs[[1]]
    -g=as.ngraph(n)
    -# find the nodes distal to this point
    -# nb you must set unreachable=F if you only want to get downstream nodes
    -igraph::dfs(g, neimode='out', unreachable = FALSE, root=n$AxonLHEP)
    +
    ## $root
     ## [1] 71
     ## 
    @@ -271,7 +271,7 @@ 

    ## [1] "out" ## ## $order -## + 180/180 vertices, named, from 2866093: +## + 180/180 vertices, named, from e0ed37b: ## [1] 72 73 74 75 76 77 78 79 80 81 82 83 84 85 ## [15] 86 87 88 89 90 91 92 93 94 95 96 97 98 99 ## [29] 100 101 102 103 104 105 106 107 108 109 110 111 112 113 @@ -292,8 +292,8 @@

    ## ## $dist ## NULL

    -
    # the proximal nodes back to the soma (including any branches)
    -igraph::dfs(g, neimode='in', unreachable = FALSE, root=n$AxonLHEP)
    +
    # the proximal nodes back to the soma (including any branches)
    +igraph::dfs(g, neimode='in', unreachable = FALSE, root=n$AxonLHEP)
    ## $root
     ## [1] 71
     ## 
    @@ -301,7 +301,7 @@ 

    ## [1] "in" ## ## $order -## + 180/180 vertices, named, from 2866093: +## + 180/180 vertices, named, from e0ed37b: ## [1] 72 71 70 69 68 67 66 65 64 63 48 47 46 45 ## [15] 44 43 34 33 32 31 30 29 28 27 26 25 24 23 ## [29] 22 21 20 19 18 17 16 15 14 13 12 11 10 9 @@ -324,14 +324,14 @@

    ## NULL

    Note that dfs (depth first search) provides a good way to visit all the nodes of the neuron

    Let’s use this to make a function that prunes neurons downstream of this axon entry point:

    -
    prune_from_lhep <- function(n, ...) {
    -  g=as.ngraph(n)
    -  downstream_indices=igraph::dfs(g, root = n$AxonLHEP, unreachable = FALSE)$order
    -  prune_vertices(n, verticestoprune = downstream_indices, invert = TRUE)
    -}
    -pruned=nlapply(Cell07PNs[1:3], prune_from_lhep)
    -plot(Cell07PNs[1:3], col='grey')
    -plot(pruned, lwd=2, add = T)
    +
    prune_from_lhep <- function(n, ...) {
    +  g=as.ngraph(n)
    +  downstream_indices=igraph::dfs(g, root = n$AxonLHEP, unreachable = FALSE)$order
    +  prune_vertices(n, verticestoprune = downstream_indices, invert = TRUE)
    +}
    +pruned=nlapply(Cell07PNs[1:3], prune_from_lhep)
    +plot(Cell07PNs[1:3], col='grey')
    +plot(pruned, lwd=2, add = T)

    The pruned neurons show up in red, green, and blue in the above plot.

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zyQF+|$rfo5y!6T5jmM{H==s1`e06f@&nE=YzI*p>xGe0>#Y75Uxx7~f3&mM&6PkI@ z=EBJ^2Ho9##nDKqhxhbkS@pv{jlyiyHnxKydABY<_sgxg)*wHr6r7#=}<1G@PwWC)n${eRAhNnq1qHk%g}@2?HN}*T^OQ2zWO{Vc8f*Y(XVe;a>O%Z z%iPvBJNDwh_#T3lCiE_NylL0}3P?WlUU~S5j%>o5QdLJGxAl(;+}}+_5r`jVzYd|+ z3_*G~ggGAim>>cgHX*lm!!8$VJ2{oYIRR;LDKPEF=2_I?gO{bGDXwb-okPTdxSQ(^ z-5T5|q&m_hp8wK7^-gtoTS#U;$j;IZlBx&f#oE;q#6IkC)Yq3kg{QI@WG0c+E9{|9 zQ@m{7OE|sg3uWCZhwqK=I^VyZRt4Dg&-`fe9~Pj^NCo1k-o-KU^bWawv%%Tq39I}0 zPhu)7C`cwFB%s)NDdWad(e0M6kO|VQq5Z>f!jx^ub6rOF)y4>8EDFD6t z96>gOp8m(6iMeK-VI5!zevk-5zoI#?6GA5`CuQP9XO($JH|eBb(s9R35(Qv?d1AH7QE043U6F6+Y_ z$$2`E%nM7aF%28gWvtPdBrmTLQ14NWk<}y;tk{eJ@OUO*b@=zWqNMScq1vyV0M(^W zMjI)`i-_N6GlKMyYTqpm-|cMe`IIniOEXnyk}ar};*>(-+UXAbp9Ato;M9 z=%w`6W95G?j9TEQPdCY-bAqY9Ke#_Q@i&?L#%WH_cBsyw3i zc`=M7a$K)vS@T&KA<`>Aw$9N+YwX~mALu`| zw6rG(OlQTJN0t#UVz%o<(2u?s&C=vrMytlOdFe^qRMUvlO!?ZgJDxD;bxwF&Aji5L z=hiOb{o|z|X#0~hCuyXd7EBM~{6|(&B7f{gaXIKA5vj1Vs;iN3?G_L)>Nq$!1foj4 zRtPoY;j&_2I)S)-SEkZd$uTPa>%dX`Q`0E@$B6?z7lV+_=q)gu1%lacplJ^)8=IgY z@AHt53un*X*l$&sBet)VaOf-ais_6+s*ZMna~ntaVt()i|NTwzYT#X-<#|B^p`5UT zJ8=KE2bC0*{lr~e`9JUs`1CdSG|@m;=zo9u_iz8r3;kWQ{}}1tb@;mue>$4K_VCvp z{@TOed-E@T_ywE(;;8?6#J_m*KgjX#I{aOS{~U?G>+p9S{>I&Z`G@}k=KmjKr^Y>M YTTbehEZ#)S4AJF nat - 1.9.0 + 1.9.1 @@ -98,7 +98,7 @@

    Introduction to neurons and neuronlists

    Gregory Jefferis

    -

    2019-01-10

    +

    2019-07-22

    Source: vignettes/neurons-intro.Rmd @@ -117,9 +117,9 @@

    Startup

    Install the package if required

    -
    install.packages('nat', dependencies = TRUE)
    +
    install.packages('nat', dependencies = TRUE)

    Load the package

    -
    library(nat)
    +
    library(nat)

    @@ -134,16 +134,16 @@

    Sample Data

    As an example we’re going to look at a data set of olfactory projection neurons originally published as Supplemental Information to accompany Jefferis, Potter et al, Cell (2007). A subset of the original data are distributed as a sample data object with the nat package, which we can load like so.

    -
    data("Cell07PNs")
    +
    data("Cell07PNs")

    For more information about these data see Cell07PNs.

    This Cell07PNs object has two classes neuronlist and the base class list

    -
    class(Cell07PNs)
    +
    class(Cell07PNs)
    ## [1] "neuronlist" "list"

    Many R objects inherit from the base class list (objects can have multiple classes in the same way that a mitral cell is also neuron) because this is a convenient container class into which you can put a variety of different kinds of data. In this case the Cell07PNs neuronlist contains 40 objects:

    -
    length(Cell07PNs)
    +
    length(Cell07PNs)
    ## [1] 40
    -
    # access the first neuron in the neuronlist
    -class(Cell07PNs[[1]])
    +
    ## [1] "neuron" "list"

    each representing a traced neuron. We will now look in detail at these two classes.

    @@ -152,16 +152,16 @@

    Neurons

    We start by extracting the first neuron in Cell07PNs, and assigning it to a variable. We can then plot the neuron so that we have an image of what we are talking about.

    -
    n1=Cell07PNs[[1]]
    -plot(n1)
    +

    Neuron internal structure

    The str function (short for structure) allows us to take a look at the internal structure of this neuron object.

    -
    # Use =1 so that we don't show complete structure
    -# for objects inside n1
    -str(n1, max.level=1)
    +
    ## List of 24
     ##  $ CellType     : chr "DA1"
     ##  $ NeuronName   : chr "EBH11R"
    @@ -191,14 +191,14 @@ 

    ## $ AxonLHEP : num 72 ## - attr(*, "class")= chr [1:2] "neuron" "list"

    From this you can see that there are a large number of fields inside the neuron. Each field can be accessed using the $ or [[ operators

    -
    n1$NumPoints
    +
    ## [1] 180
    -
    n1[["NumPoints"]]
    +
    ## [1] 180

    There are a set of core fields (described in ?neuron documentation); the key ones will be described shortly. However there are also quite a few user fields in this neuron and you can safely add any field you like so long as its name does not clash with an existing field. For example:

    -
    n1$Comment='The sex of this specimen is uncertain'
    +

    The single most important field in a neuron is the $d data.frame. This contains a block of data closely reminiscent of the SWC data format for traced neurons:

    -
    str(n1$d)
    +
    str(n1$d)
    ## 'data.frame':    180 obs. of  7 variables:
     ##  $ PointNo: int  1 2 3 4 5 6 7 8 9 10 ...
     ##  $ Label  : num  2 2 2 2 2 2 2 2 2 2 ...
    @@ -215,10 +215,10 @@ 

    R’s simple S3 object oriented system allows specialised functions for particular classes to be defined. These functions tailored to a particular class are called methods. Methods can be provided for pre-existing functions supplied with base R as well as new user-written functions. The system is quite simple. If a function foo is defined as a generic function then you can define new functions called foo.bar that will be called when you write foo(x) and x has class `bar.

    We already used one such method without comment, plot.neuron. There is a base R function called plot. nat defines a new function called plot.neuron, which R interprets as a plot method specialised for neuron objects. When the base R plot function is called, it looks at the class of its first object to see whether a specialised method exists. If there is none, it will use a fallback method called plot.default.

    The plot.neuron method interprets the branching structure of a neuron and draws line segments joining up the connected nodes at the appropriate 2D positions. You can compare it with what you would get if you just plotted the XY position of all nodes joined together by a single line:

    -
    plot(n1$d$X, n1$d$Y, type = 'l')
    +
    plot(n1$d$X, n1$d$Y, type = 'l')

    You can find out what methods are available for a particular class like so:

    -
    methods(class = 'neuron')
    +
    methods(class = 'neuron')
    ##  [1] all.equal          as.neuron          as.ngraph         
     ##  [4] as.seglist         branchpoints       dotprops          
     ##  [7] endpoints          intersect_plane    ndigest           
    @@ -231,26 +231,26 @@ 

    You can then find the help page for any method in the console with ?plot.neuron. Note that if you write ?plot you will get the documentation for the basic plot function supplied with R.

    It is also good idea to look at nat’s function reference page which groups available functions into categories that often reflect the class of object they can work on.

    In R, operators such as * or + are actually special functions with two arguments, so one can add methods for these in the same way. In the following example we use this arithmetic to shift a neuron by a small amount:

    -
    plot(n1, col = 'black', WithNodes = F, main="Shifting neurons")
    -# shift by 3 microns in x,y,z
    -plot(n1+3, col = 'red', add = TRUE, WithNodes = F)
    -# shift by -5 microns in y
    -plot(n1+c(0,-5,0), col = 'blue', add = TRUE, WithNodes = F)
    +
    plot(n1, col = 'black', WithNodes = F, main="Shifting neurons")
    +# shift by 3 microns in x,y,z
    +plot(n1+3, col = 'red', add = TRUE, WithNodes = F)
    +# shift by -5 microns in y
    +plot(n1+c(0,-5,0), col = 'blue', add = TRUE, WithNodes = F)

    Another specialised method is subset.neuron which we can use to extract part of a neuron into a new object. For example, a simple spatial criterion, X location must be >240, is used to extract the axon terminal arborisation field in this example

    -
    plot(n1, col = 'black', WithAllPoints = T, main="Subsetting a neuron")
    -# keep only nodes with X position >20
    -n1.lh=subset(n1, X>240)
    -# plot the selected part of the neuron in blue
    -plot(n1.lh, col='blue', lwd=3, add=TRUE)
    -# add a line illustrating the cut point
    -abline(v=240, lty=2)
    +
    plot(n1, col = 'black', WithAllPoints = T, main="Subsetting a neuron")
    +# keep only nodes with X position >20
    +n1.lh=subset(n1, X>240)
    +# plot the selected part of the neuron in blue
    +plot(n1.lh, col='blue', lwd=3, add=TRUE)
    +# add a line illustrating the cut point
    +abline(v=240, lty=2)

    We can also summarise the morphological properties of a neuron using the summary.neuron method, which allows us to compare measurements for the original neuron and the axon arbour that we just cut out above.

    - +
    ##   root nodes segments branchpoints endpoints cable.length
     ## 1    1   180       33           16        18     297.1763
    -
    summary(n1.lh)
    +
    summary(n1.lh)
    ##   root nodes segments branchpoints endpoints cable.length
     ## 1    1   111       27           13        15     156.7654

    See nat’s function reference page for additional functions for working with neurons.

    @@ -274,9 +274,9 @@

    Worked Examples

    Let us start by extracting the first five neurons from our sample data.

    -
    # we can select neurons by indexing 
    -first5=Cell07PNs[1:5]
    -summary(first5)
    +
    ##        root nodes segments branchpoints endpoints cable.length
     ## EBH11R    1   180       33           16        18     297.1763
     ## EBH20L    1   200       26           12        15     327.0929
    @@ -284,15 +284,15 @@ 

    ## EBI12L 1 169 23 11 13 294.4680 ## EBI22R 1 160 27 13 15 303.0150

    Each neuron in the neuronlist has an associated name which can be used to select it. We can get the names of all neurons using the names function.

    -
    names(Cell07PNs)
    +
    names(Cell07PNs)
    ##  [1] "EBH11R" "EBH20L" "EBH20R" "EBI12L" "EBI22R" "EBJ23L" "EBJ3R" 
     ##  [8] "EBN19L" "EBO15L" "EBO53L" "ECA34L" "ECB3L"  "LI23L"  "LIC2R" 
     ## [15] "LJ5L"   "MC3B"   "MH16L"  "MM14L"  "NA7L"   "NH15L"  "NH29B" 
     ## [22] "NI16L"  "NIA8L"  "NIA8R"  "NNA9L"  "NNC4R"  "NNE1L"  "OFD2L" 
     ## [29] "OKC9R"  "SDD8L"  "SH21L"  "SL20L"  "TKC8R"  "TL4R"   "TS7L"  
     ## [36] "TT27R"  "VA15R"  "VA20R"  "VB37L"  "VB58L"
    -
    nl3=Cell07PNs[c("EBH11R", "EBH20L", "EBH20R")]
    -all.equal(nl3, Cell07PNs[1:3])
    +
    nl3=Cell07PNs[c("EBH11R", "EBH20L", "EBH20R")]
    +all.equal(nl3, Cell07PNs[1:3])
    ## [1] TRUE

    You can also use the $ operator to access a single neuron e.g. Cell07PNs$EBH11R. This can be quite useful when working interactively in the console because RStudio will offer to autocomplete the neuron name when you start typing past the $ symbol, but is not recommended for scripts.

    @@ -300,7 +300,7 @@

    Metadata

    The names of neurons are also used to index a data.frame object attached to the neuronlist with one row for each neuron. You can use the head function to give a summary of the attached metadata in this neuronlist. Using the as.data.frame method on a neuronlist allows you to extract this attached metadata to a separate object.

    -
    head(Cell07PNs)
    +
    head(Cell07PNs)
    ##         Brain RegistrationScore Notes Glomerulus Batch Directory Traced
     ## EBH11R EBH11R                 4              DA1   PN2    unsure    yes
     ## EBH20L EBH20L                 4              DL3   PN2   unsure2    yes
    @@ -343,11 +343,11 @@ 

    ## EBI12L /GD/projects/PN2/allreg/warp/unsure3/average-goodbrains_EBI12L101_warp_m0g40c4e1e-1x16r3.list ## EBI22R /GD/projects/PN2/allreg/warp/unsure3/average-goodbrains_EBI22R101_warp_m0g40c4e1e-1x16r3.list ## EBJ23L /GD/projects/PN2/allreg/warp/unsure4/average-goodbrains_EBJ23L101_warp_m0g40c4e1e-1x16r3.list

    -
    df=as.data.frame(Cell07PNs)
    -nrow(df)
    +
    df=as.data.frame(Cell07PNs)
    +nrow(df)
    ## [1] 40
    -
    # rownames of data.frame are the same as names of Cell07PNs list.
    -all.equal(rownames(df), names(Cell07PNs))
    +
    ## [1] TRUE

    You can use columns in the attached metadata in expressions that select or operate on the neurons in the list. In the next two examples we

      @@ -355,35 +355,35 @@

      subset the neurons in a neuronlist based on a metadata column
    • use the with function to carry out a calculation using column(s) of metadata.
    -
    # subset.neuronlist method (which you call using the subset function)
    -da1n=subset(Cell07PNs, Glomerulus=="DA1")
    -
    -# with.neuronlist method
    -with(Cell07PNs, table(Glomerulus))
    +
    ## Glomerulus
     ##  DA1  DL3 DP1m VA1d 
     ##   11   10    8   11

    There is a second approach to accessing this attached metadata data.frame, using the square bracket operator with two arguments as used for subscripting (2D) matrices or data.frames. For example we can access the Glomerulus column like so:

    -
    Cell07PNs[, 'Glomerulus']
    +
    ##  [1] DA1  DL3  DA1  DA1  DL3  DL3  VA1d VA1d VA1d VA1d DP1m DP1m DA1  DL3 
     ## [15] VA1d VA1d DL3  DA1  DA1  VA1d VA1d VA1d DL3  VA1d DP1m DP1m DP1m DP1m
     ## [29] DP1m DP1m DA1  DL3  DL3  DL3  VA1d DA1  DA1  DA1  DL3  DA1 
     ## Levels: DA1 DL3 DP1m VA1d

    This approach has some features that make for additional flexibility. For example you can access the whole data.frame like so:

    -
    summary(Cell07PNs[,])
    +
    summary(Cell07PNs[,])

    The form [,] which includes a comma implies two (missing) arguments. nat therefore interprets this as request for the 2D data.frame attached to Cell07PNs rather than the neuronlist itself. You can also use this notation to add new columns or modify existing columns in place.

    -
    Cell07PNs[, 'NewColumn'] = somevariable
    -Cell07PNs[, "Sex"] = sub("F", "female", Cell07PNs[, "Sex"])
    -Cell07PNs[, "Sex"] = sub("M", "male", Cell07PNs[, "Sex"])
    +
    Cell07PNs[, 'NewColumn'] = somevariable
    +Cell07PNs[, "Sex"] = sub("F", "female", Cell07PNs[, "Sex"])
    +Cell07PNs[, "Sex"] = sub("M", "male", Cell07PNs[, "Sex"])

    You can also access selected rows of the data.frame in the normal way:

    -
    Cell07PNs[1:5, "Glomerulus"]
    -Cell07PNs[Cell07PNs[, "Sex"]=="F", "Glomerulus"]
    +

    Working on all the neurons in a neuronlist

    There are quite a few methods defined for the neuronlist class:

    -
    methods(class = "neuronlist")
    +
    methods(class = "neuronlist")
    ##  [1] -                  [                  [<-               
     ##  [4] *                  /                  +                 
     ##  [7] as.data.frame      as.neuronlistfh    c                 
    @@ -396,16 +396,16 @@ 

    ## [28] xform xyzmatrix xyzmatrix<- ## see '?methods' for accessing help and source code

    For example we can scale the position (and diameter) of all of the neurons in a neuronlist using the * operator:

    -
    # convert from microns to nm
    -Cell07PNs.nm = Cell07PNs*1e3
    -plot(Cell07PNs.nm)
    +

    There is also a more powerful xform function that allows arbitrary transformations to be applied. For example here we apply a 3D rotation expressed as a homogeneous affine matrix:

    -
    # define a 180 degree rotation around the x axis
    -rotm=cmtkparams2affmat(rx=180)
    -# remove tiny values due to rounding errors
    -rotm=zapsmall(rotm)
    -plot(xform(Cell07PNs, rotm))
    +

    More generally any function that works on a neuron can be applied to a neuronlist by using the function nlapply. This is an analogue to base R’s lapply function. This will take care of returning a new neuronlist with the attached metadata and includes additional features such as error tolerance, progress bars and parallelisation for multi-core machines. It is worth reviewing the help and examples for this function carefully if you start to work regularly with neuronlists.

    @@ -414,26 +414,26 @@

    Plots

    We have already seen that we can plot a single neuron (the first) like so:

    -
    plot(Cell07PNs[[1]])
    +
    plot(Cell07PNs[[1]])

    The purple node highlights the root or soma of the neuron, red nodes are branch points, green nodes are end points. We can also label each node with its PointNo index.

    -
    plot(Cell07PNs[[1]], col='red', WithText=T)
    +
    plot(Cell07PNs[[1]], col='red', WithText=T)

    Multiple neurons can be plotted by passing a whole neuronlist or indexing it

    -
    plot(Cell07PNs[1:3])
    +
    plot(Cell07PNs[1:3])

    More complex subsets are possible by using plot.neuronlist subset argument (which works in the same way as the subset.neuronlist function). For example here we select neurons by which olfactory glomeruli their dendrites occupy:

    -
    plot(Cell07PNs, subset=Glomerulus!="DA1", col='grey', WithNodes=F, main="DA1 neurons")
    -plot(Cell07PNs, subset=Glomerulus=="DA1", add=TRUE)
    +
    plot(Cell07PNs, subset=Glomerulus!="DA1", col='grey', WithNodes=F, main="DA1 neurons")
    +plot(Cell07PNs, subset=Glomerulus=="DA1", add=TRUE)

    3D plots

    So far we have only used 2D plots in this document, but especially for interactive analysis and exploration, it is much more helpful to have 3D plots that can be rotated, zoomed etc. nat provides numerous functions for 3D plots based on the rgl package. It is actually possible to embed fully interactive 3d figures in rmarkdown reports like this one by setting the webgl chunk option.

    -
    clear3d()
    -plot3d(Cell07PNs[[1]], col='red')
    -# set a grey background so it's easier to see extent of the webgl canvas
    -bg3d(col='lightgrey')
    +

    You must enable Javascript to view this page properly.

    You should be able to rotate (click and drag) and zoom (mouse wheel) this figure.

    Since this results in quite large html files when used with many neurons, we will not use it further here. For now let’s show a plot in which we both select a subset of neurons and colour them according to their glomerulus of origin.

    -
    # 3d plot of neurons from olfactory glomeruli beginning with a D
    -# coloured by glomerulus
    -rval=plot3d(Cell07PNs, subset=grepl("^D", Glomerulus), col=Glomerulus,
    -  lwd=2, WithNodes=FALSE)
    +

    -
    # make a legend so that you know which colours match which glomerulus
    -plot.new()
    -with(attr(rval,'df'), legend('center', legend = unique(Glomerulus), fill=unique(col)))
    +

    @@ -4720,33 +4819,33 @@

    Putting it all together

    To close this tutorial, let’s do some morphological analysis of the neurons in this dataset. There are second order olfactory projection neurons originating from 4 different glomeruli in the Cell07PNs sample dataset, each coming from a different animal. We can ask whether their axon terminal arborisations in a brain area called the lateral horn show any features that are distinctive for different classes. This would be consistent with the hypothesis that information from different glomeruli (i.e. olfactory channels) is handled in a stereotyped way in this brain area.

    Let’s start by cutting out the arbour within the lateral horn from all of the neurons. We’ll use a very simple approach of cutting at X=250, although nat has more sophisticated ways to do this e.g. by using 3D spaces defined by a surface mesh.

    -
    # note that we had to use OmitFailures=T due to a problem with the graph
    -# structure of one neuron
    -lha=nlapply(Cell07PNs, subset, X>250, OmitFailures = T)
    -# Let's plot what we have colouring by glomerulus
    -plot(lha, col=Glomerulus, WithNodes=F)
    +

    Now we can get a basic statistical summary by axon arbour.

    -
    lhstats=summary(lha, include.attached.dataframe = TRUE)
    -boxplot(lhstats$cable.length~lhstats$Glomerulus, log='y')
    +
    lhstats=summary(lha, include.attached.dataframe = TRUE)
    +boxplot(lhstats$cable.length~lhstats$Glomerulus, log='y')

    -
    boxplot(lhstats$branchpoints~lhstats$Glomerulus)
    +
    boxplot(lhstats$branchpoints~lhstats$Glomerulus)

    We can also calculate the median position of the arbour for each neuron and add that to the lhstats database

    -
    # quick function to calculate centroid
    -arbour_centroid <- function(n) {
    -  # extract location of all points in neuron object
    -  xyzs=xyzmatrix(n)
    -  # median in all 3 axes (=>2 columns)
    -  apply(xyzs, 2, median)
    -}
    -# note that we have to transpose because sapply results in x,y,z rows
    -centroids=t(sapply(lha, arbour_centroid))
    -lhstats=cbind(lhstats, centroids)
    +

    Finally we can take these data and see how well we can predict the identity of the neuron (i.e. its glomerulus) based on the statistics of the axon arbour in the lateral horn. We use the linear discriminant analysis (provided by the function lda in recommended package MASS). This also runs a leave one out cross-validation to get a more robust initial estimate of prediction error.

    -
    library(MASS)
    -lda.fit = lda(Glomerulus ~ cable.length+X+Y+Z+branchpoints, lhstats, CV = T)
    -table(lda.fit$class, lhstats$Glomerulus)
    +
    library(MASS)
    +lda.fit = lda(Glomerulus ~ cable.length+X+Y+Z+branchpoints, lhstats, CV = T)
    +table(lda.fit$class, lhstats$Glomerulus)
    ##       
     ##        DA1 DL3 DP1m VA1d
     ##   DA1   10   2    0    1
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    diff --git a/docs/authors.html b/docs/authors.html
    index 13a95445..e8b49638 100644
    --- a/docs/authors.html
    +++ b/docs/authors.html
    @@ -60,7 +60,7 @@
           
           
             nat
    -        1.9.0
    +        1.9.1
           
         
     
    @@ -133,7 +133,7 @@ 

    Citation

    Jefferis, Gregory S X E, Manton, James D (2017). “NeuroAnatomy Toolbox.” ZENODO. -doi: 10.5281/zenodo.598047. +doi: 10.5281/zenodo.598047.

    @Article{,
       title = {{NeuroAnatomy Toolbox}},
    diff --git a/docs/index.html b/docs/index.html
    index 030d568e..0a3bf73b 100644
    --- a/docs/index.html
    +++ b/docs/index.html
    @@ -40,7 +40,7 @@
           
           
             nat
    -        1.9.0
    +        1.9.1
           
         
     
    @@ -114,29 +114,29 @@
     

    Quick Start

    For the impatient …

    -
    # install
    -install.packages("nat")
    -# use
    -library(nat)
    -
    -# plot some test data (?kcs20 for details)
    -# Drosophila Kenyon cells processed from raw data at http://flycircuit.tw
    -head(kcs20)
    -open3d()
    -plot3d(kcs20, col=type)
    -# get help
    -?nat
    +

    Installation

    A confirmed stable version of nat can be installed from CRAN.

    - +

    However, nat remains under quite active development, so if you will be using nat extensively, we generally recommend installing the latest development version directly from github using the devtools package.

    -
    # install devtools if required
    -if (!requireNamespace("devtools")) install.packages("devtools")
    -# then install nat
    -devtools::install_github("jefferis/nat")
    +

    @@ -152,15 +152,21 @@

  • Read the overview package documentation (?nat in R)
  • Read the Introduction to neurons
  • -
  • Check out the thematically organised function reference documentation.
  • +
  • Check out the thematically organised function reference documentation. +
    • Most help pages include examples.
    • -
    • Try out sample code:
    • +
    +
  • +
  • Try out sample code: + +
  • +

    @@ -174,11 +180,14 @@

    If you think that you have found a bug:

    • Install the development version of nat using devtools (see above) and see if that helps.
    • -
    • Check the github issues and
    • +
    • Check the github issues and +
      • file a bug report if this seems to be a new problem
      • comment on an existing bug report
      +
    • +

    Thanks for your interest in nat!

    diff --git a/docs/news/index.html b/docs/news/index.html index 4806c517..420f30e2 100644 --- a/docs/news/index.html +++ b/docs/news/index.html @@ -60,7 +60,7 @@ nat - 1.9.0 + 1.9.1 @@ -130,6 +130,14 @@

    Changelog

    Source: NEWS.md +
    +

    +nat 1.9.1 Unreleased +

    +
      +
    • Fix very slow loading of neurons in neuronlistfh() objects. Apparently due to a change in behaviour in R 3.6.0 (#402). Thanks to Shanice Bailey and Lisa Marin for the bug report.
    • +
    +

    nat 1.9.0 Unreleased @@ -419,7 +427,7 @@

  • plot.neuronlist sets axes based on boundingbox of all plotted neurons, not just the first neuron (#223)
  • plot.neuron(,AxisDirections) argument is re-implemented (#224)
  • don’t warn when read.neuronlistfh changes the directory location on disk (#157)
  • -
  • remove superimposed plots in mirror example (hope this will fix build on macosx snowleopard)
  • +
  • remove superimposed plots in mirror example (hope this will fix build on MacOS X Snow Leopard)
  • teach read.neurons to OmitFailures when reading in a neuronlistfh object.
  • fix handling of default colours in plot.neuronlist and plot3d.neuronlist (#230)
  • fix plot3d.neuronlist returns one element per neuron
  • @@ -499,7 +507,7 @@

    • add nlscan function to review a set of neurons, optionally selecting a subset
    • -
    • add find.soma function to select neurons whose cell bodies fall within an rgl seelection box (thanks to Ben Sutcliffe).
    • +
    • add find.soma function to select neurons whose cell bodies fall within an rgl selection box (thanks to Ben Sutcliffe).
    • add setdiff, intersect and union generics with implementations for neuronlist objects (note this is done in more or less identical fashion to generics defined in the dplyr package).
    • give write.neurons a format argument to make it more obvious that this is an option rather than pointing people to the write.neuron docs (suggestion from Ben Sutcliffe)
    • teach as.neuronlist to handle inputs with empty (rather than NULL names)
    • @@ -531,7 +539,7 @@

    • c.neuronlist can now join lists with attached data.frames with different columns (missing values are filled with NAs). This also applies when some neuronlists have no attached data.frame at all.
    • read.neurons can read neuronlist files from disk
    • cmtk.statistics now has a Verbose argument
    • -
    • warn if resmapling a neuron with multiple subtrees that only the main subtree will be kept.
    • +
    • warn if resampling a neuron with multiple subtrees that only the main subtree will be kept.
    @@ -596,7 +604,7 @@

    • give mirror/xform.neuronlist subset arguments
    • add plot3d.boundingbox
    • -
    • give plot(3d).neuronlist a SUBSTITUTE argument that can be used to control non-stadard evaluation. This is useful for people who wish to incoroporate these funcitons inside other user-defined functions. See ?subset.neuronlist for details.
    • +
    • give plot(3d).neuronlist a SUBSTITUTE argument that can be used to control non-standard evaluation. This is useful for people who wish to incorporate these functions inside other user-defined functions. See ?subset.neuronlist for details.
    • fix: write.neurons into subdirs when df=NULL

    @@ -625,7 +633,7 @@

    nat 1.5.3 Unreleased

      -
    • new function pointsinside to determine if points (e.g. neuron) are inside a closedd surface
    • +
    • new function pointsinside to determine if points (e.g. neuron) are inside a closed surface
    • resample.neuron implemented (no support for interpolating widths or multiple subtrees)
    • dotprops.neuron can resample to a consistent segment length
    • consistent return values for as.seglist and seglengths when all subtrees are returned
    • @@ -647,7 +655,7 @@

    • always drop unused vertices in as.mesh3d.hxsurf
    • fix bug in write.neurons when subdir not specified
    • fix colouring of vectors by plot3d.dotprops
    • -
    • dev: switch to roxygygen2 v4
    • +
    • dev: switch to roxygen2 v4
    @@ -677,7 +685,7 @@

  • add xyzmatrix method for neuronlists
  • add materials function with methods for im3d and hxsurf objects
  • enh: read.im3d can read materials from amiramesh files
  • -
  • enh: download neurnonlistfh objects to session temp directory if no localdir is specified
  • +
  • enh: download neuronlistfh objects to session temp directory if no localdir is specified
  • fix: find CMTK in its new default location on macosx
  • fix: plot3d should skip redrawing if par3d() skipRedraw is set
  • fix: failure to export ngraph constructor function
  • @@ -904,7 +912,7 @@

    • implement read.neuron and read.neurons
    • so far only works for rda, rds and swc files
    • -
    • reading of additional file types will be implemented by registering functions = to test, read and write for the format using a package regsistry controlled by nat::neuronformats.
    • +
    • reading of additional file types will be implemented by registering functions = to test, read and write for the format using a package registry controlled by nat::neuronformats.
    • developer: seglists in neurons now have class ‘seglist’

    @@ -941,7 +949,7 @@

  • teach mirror function to accept numeric axis specification
  • Fix bug in checking extra directories in cmtk.bindir
  • Add dotprops.neuronlist and include all dotprops methods in documentation
  • -
  • Fix longstanding bug in parsing cmtk warping registrations (only relevant for reading these into R lists - no effect on transformations).
  • +
  • Fix long-standing bug in parsing cmtk warping registrations (only relevant for reading these into R lists - no effect on transformations).
  • fix bug in class of cmtkreg objects
  • Remove call to .Internal(La_rs) by calling eigen directly
  • doc: some notes on CMTK installation
  • @@ -979,7 +987,7 @@

  • basic and self-contained implementation of dotprops
  • new xform generic + methods for clean transformation of objects including neurons and dotprops objects
  • this includes improved interaction between dotprops and xform so that xform can use the previously value of k when recalculating a dotprops object post transformation
  • -
  • sample data (40 PNs from Jefferis, Potter et al. ’07 and 20 KCs from Chiang et al. 2011)
  • +
  • sample data (40 PNs from Jefferis, Potter et al. ’07 and 20 KCs from Chiang et al. 2011)
  • 1 use of .Internal and missing nlapply remain to be fixed.
  • @@ -1008,6 +1016,7 @@

    Contents

    diff --git a/docs/reference/MBL.surf.html b/docs/reference/MBL.surf.html index 679a3d29..a74cbbcb 100644 --- a/docs/reference/MBL.surf.html +++ b/docs/reference/MBL.surf.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/affmat2cmtkparams.html b/docs/reference/affmat2cmtkparams.html index 4a85fe19..718187d0 100644 --- a/docs/reference/affmat2cmtkparams.html +++ b/docs/reference/affmat2cmtkparams.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/all.equal.dotprops.html b/docs/reference/all.equal.dotprops.html index 82614d1b..7772b4df 100644 --- a/docs/reference/all.equal.dotprops.html +++ b/docs/reference/all.equal.dotprops.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/all.equal.im3d.html b/docs/reference/all.equal.im3d.html index f69f96f3..138b158d 100644 --- a/docs/reference/all.equal.im3d.html +++ b/docs/reference/all.equal.im3d.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/all.equal.neuron.html b/docs/reference/all.equal.neuron.html index 7befe53a..205b2e78 100644 --- a/docs/reference/all.equal.neuron.html +++ b/docs/reference/all.equal.neuron.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/amiramesh-io.html b/docs/reference/amiramesh-io.html index 18da123f..ab0d5989 100644 --- a/docs/reference/amiramesh-io.html +++ b/docs/reference/amiramesh-io.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/amiratype.html b/docs/reference/amiratype.html index 1a769b6d..f6352a0e 100644 --- a/docs/reference/amiratype.html +++ b/docs/reference/amiratype.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/as.hxsurf.html b/docs/reference/as.hxsurf.html index e2c2cfe7..d0c5e510 100644 --- a/docs/reference/as.hxsurf.html +++ b/docs/reference/as.hxsurf.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/as.im3d.html b/docs/reference/as.im3d.html index 2c4f55be..117ec556 100644 --- a/docs/reference/as.im3d.html +++ b/docs/reference/as.im3d.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/as.mesh3d.html b/docs/reference/as.mesh3d.html index 59835ae2..10a4a480 100644 --- a/docs/reference/as.mesh3d.html +++ b/docs/reference/as.mesh3d.html @@ -69,7 +69,7 @@ nat - 1.9.0 + 1.9.1 @@ -213,7 +213,7 @@

    Details

    See also

    ashape3d, mesh3d

    -

    as.mesh3d, tmesh3d, +

    as.mesh3d, tmesh3d, as.hxsurf, read.hxsurf

    Other hxsurf: as.hxsurf, materials, plot3d.hxsurf, @@ -222,12 +222,8 @@

    See a

    Examples

    -
    library(alphashape3d)
    #> Loading required package: geometry
    #> Loading required package: magic
    #> Loading required package: abind
    kcs20.a=ashape3d(xyzmatrix(kcs20), alpha = 10)
    #> -#> PLEASE NOTE: As of version 0.3-5, no degenerate (zero area) -#> regions are returned with the "Qt" option since the R -#> code removes them from the triangulation. -#> See help("delaunayn"). -#>
    plot(kcs20.a)
    #> Device 1 : alpha = 10
    +
    library(alphashape3d)
    #> Loading required package: geometry
    kcs20.a=ashape3d(xyzmatrix(kcs20), alpha = 10) +plot(kcs20.a)
    #> Device 1 : alpha = 10
    # convert to mesh3d kcs20.mesh=as.mesh3d(kcs20.a) diff --git a/docs/reference/as.neuronlist.html b/docs/reference/as.neuronlist.html index 98998d10..325a6525 100644 --- a/docs/reference/as.neuronlist.html +++ b/docs/reference/as.neuronlist.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1
    diff --git a/docs/reference/as.neuronlist.neuronlistfh.html b/docs/reference/as.neuronlist.neuronlistfh.html index 013ed856..1b344108 100644 --- a/docs/reference/as.neuronlist.neuronlistfh.html +++ b/docs/reference/as.neuronlist.neuronlistfh.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1
    diff --git a/docs/reference/boundingbox.html b/docs/reference/boundingbox.html index e080677f..691ff562 100644 --- a/docs/reference/boundingbox.html +++ b/docs/reference/boundingbox.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1
    diff --git a/docs/reference/c.neuronlist.html b/docs/reference/c.neuronlist.html index 1acf45e4..6381133d 100644 --- a/docs/reference/c.neuronlist.html +++ b/docs/reference/c.neuronlist.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/clampmax.html b/docs/reference/clampmax.html index 7e710632..b64c6ca8 100644 --- a/docs/reference/clampmax.html +++ b/docs/reference/clampmax.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtk.bindir.html b/docs/reference/cmtk.bindir.html index 0cd63f85..24350ddb 100644 --- a/docs/reference/cmtk.bindir.html +++ b/docs/reference/cmtk.bindir.html @@ -67,7 +67,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtk.call.html b/docs/reference/cmtk.call.html index fe67db42..84535222 100644 --- a/docs/reference/cmtk.call.html +++ b/docs/reference/cmtk.call.html @@ -66,7 +66,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtk.dof2mat.html b/docs/reference/cmtk.dof2mat.html index 092c5c6f..0f06c4e0 100644 --- a/docs/reference/cmtk.dof2mat.html +++ b/docs/reference/cmtk.dof2mat.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtk.extract_affine.html b/docs/reference/cmtk.extract_affine.html index 7495348a..b18daf29 100644 --- a/docs/reference/cmtk.extract_affine.html +++ b/docs/reference/cmtk.extract_affine.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtk.mat2dof.html b/docs/reference/cmtk.mat2dof.html index 8d5c55db..29d1e4b5 100644 --- a/docs/reference/cmtk.mat2dof.html +++ b/docs/reference/cmtk.mat2dof.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtk.reformatx.html b/docs/reference/cmtk.reformatx.html index ef729dbd..01d2e262 100644 --- a/docs/reference/cmtk.reformatx.html +++ b/docs/reference/cmtk.reformatx.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtk.statistics.html b/docs/reference/cmtk.statistics.html index 51367fb7..8f6429b2 100644 --- a/docs/reference/cmtk.statistics.html +++ b/docs/reference/cmtk.statistics.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtk.targetvolume.html b/docs/reference/cmtk.targetvolume.html index a992ee51..1dea7a53 100644 --- a/docs/reference/cmtk.targetvolume.html +++ b/docs/reference/cmtk.targetvolume.html @@ -66,7 +66,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtk.version.html b/docs/reference/cmtk.version.html index b2bc8ae6..6fd46cf2 100644 --- a/docs/reference/cmtk.version.html +++ b/docs/reference/cmtk.version.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtkparams2affmat.html b/docs/reference/cmtkparams2affmat.html index 9a00d195..98c24c63 100644 --- a/docs/reference/cmtkparams2affmat.html +++ b/docs/reference/cmtkparams2affmat.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtkreg.html b/docs/reference/cmtkreg.html index a38c5c9f..2aa531b7 100644 --- a/docs/reference/cmtkreg.html +++ b/docs/reference/cmtkreg.html @@ -71,7 +71,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/cmtkreglist.html b/docs/reference/cmtkreglist.html index ba0e4119..e6430886 100644 --- a/docs/reference/cmtkreglist.html +++ b/docs/reference/cmtkreglist.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/coord2ind.html b/docs/reference/coord2ind.html index d130bf01..f199d012 100644 --- a/docs/reference/coord2ind.html +++ b/docs/reference/coord2ind.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/dotprops-arithmetic.html b/docs/reference/dotprops-arithmetic.html index 183cac9a..88f3a94d 100644 --- a/docs/reference/dotprops-arithmetic.html +++ b/docs/reference/dotprops-arithmetic.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/dotprops.html b/docs/reference/dotprops.html index 351a27b3..e5f6efb6 100644 --- a/docs/reference/dotprops.html +++ b/docs/reference/dotprops.html @@ -70,7 +70,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/fileformats.html b/docs/reference/fileformats.html index 65091787..20f202f0 100644 --- a/docs/reference/fileformats.html +++ b/docs/reference/fileformats.html @@ -68,7 +68,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/find.neuron.html b/docs/reference/find.neuron.html index e7e0ac63..0a1c7eae 100644 --- a/docs/reference/find.neuron.html +++ b/docs/reference/find.neuron.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/find.soma.html b/docs/reference/find.soma.html index 934f4d0f..c037f6f9 100644 --- a/docs/reference/find.soma.html +++ b/docs/reference/find.soma.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/flip.html b/docs/reference/flip.html index b9e0e1ea..9de50086 100644 --- a/docs/reference/flip.html +++ b/docs/reference/flip.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/get-set-neuronlist-data.frame.html b/docs/reference/get-set-neuronlist-data.frame.html index a08c162f..0c196c19 100644 --- a/docs/reference/get-set-neuronlist-data.frame.html +++ b/docs/reference/get-set-neuronlist-data.frame.html @@ -67,7 +67,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/graph.nodes.html b/docs/reference/graph.nodes.html index a4ce7184..4dc47ead 100644 --- a/docs/reference/graph.nodes.html +++ b/docs/reference/graph.nodes.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 @@ -148,7 +148,7 @@

    Arg x -

    An igraph (or ngraph) object

    +

    An ngraph or raw igraph object

    type @@ -161,7 +161,7 @@

    Arg exclude.isolated -

    Do not count isolated vertices as root points +

    Do not count isolated vertices as root/end points (default)

    @@ -182,8 +182,10 @@

    See a

    Examples

    ng=as.ngraph(Cell07PNs[[1]]) -# arbitrary vertex identifiers -graph.nodes(ng, type = 'end')
    #> [1] 1 42 59 62 80 85 96 100 102 112 117 121 134 148 154 165 172 180
    # raw vertex indices +# set some arbitrary vertex identifiers +igraph::vertex_attr(ng, 'name') <-sample(500, nvertices(ng)) +# return those identifiers +graph.nodes(ng, type = 'end')
    #> [1] 63 266 264 364 258 12 241 97 288 361 309 171 456 85 322 256 49 434
    # ... or raw vertex indices graph.nodes(ng,type = 'end', original.ids = FALSE)
    #> [1] 1 42 59 62 80 85 96 100 102 112 117 121 134 148 154 165 172 180
    diff --git a/docs/reference/im3d-io.html b/docs/reference/im3d-io.html index 1539ccf2..9fec10e0 100644 --- a/docs/reference/im3d-io.html +++ b/docs/reference/im3d-io.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/im3d.html b/docs/reference/im3d.html index c0d3ab82..abd7fd43 100644 --- a/docs/reference/im3d.html +++ b/docs/reference/im3d.html @@ -68,7 +68,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/image.im3d.html b/docs/reference/image.im3d.html index 3c8e1bda..7087d07d 100644 --- a/docs/reference/image.im3d.html +++ b/docs/reference/image.im3d.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/imexpand.grid.html b/docs/reference/imexpand.grid.html index b5fd7b0f..1fb733af 100644 --- a/docs/reference/imexpand.grid.html +++ b/docs/reference/imexpand.grid.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/imscalebar.html b/docs/reference/imscalebar.html index eac32328..edb96254 100644 --- a/docs/reference/imscalebar.html +++ b/docs/reference/imscalebar.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/imslice.html b/docs/reference/imslice.html index 2b7ab036..1e7af677 100644 --- a/docs/reference/imslice.html +++ b/docs/reference/imslice.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/ind2coord.html b/docs/reference/ind2coord.html index e527c5ba..745b28e6 100644 --- a/docs/reference/ind2coord.html +++ b/docs/reference/ind2coord.html @@ -66,7 +66,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/index.html b/docs/reference/index.html index 6e2246bf..92fd281c 100644 --- a/docs/reference/index.html +++ b/docs/reference/index.html @@ -60,7 +60,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/intersect.html b/docs/reference/intersect.html index ed4f1749..429b892f 100644 --- a/docs/reference/intersect.html +++ b/docs/reference/intersect.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/intersect_plane.html b/docs/reference/intersect_plane.html index f04223ea..596af90e 100644 --- a/docs/reference/intersect_plane.html +++ b/docs/reference/intersect_plane.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/is.amiramesh.html b/docs/reference/is.amiramesh.html index fa000a41..05c6fb04 100644 --- a/docs/reference/is.amiramesh.html +++ b/docs/reference/is.amiramesh.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/is.fijitraces.html b/docs/reference/is.fijitraces.html index 8728e845..5536d9ad 100644 --- a/docs/reference/is.fijitraces.html +++ b/docs/reference/is.fijitraces.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/is.im3d.html b/docs/reference/is.im3d.html index 6f86daaf..79c6c1db 100644 --- a/docs/reference/is.im3d.html +++ b/docs/reference/is.im3d.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/is.neuroml.html b/docs/reference/is.neuroml.html index 4887f1e5..4887c581 100644 --- a/docs/reference/is.neuroml.html +++ b/docs/reference/is.neuroml.html @@ -66,7 +66,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/is.neuronlist.html b/docs/reference/is.neuronlist.html index 9cecc7ca..ce1472ad 100644 --- a/docs/reference/is.neuronlist.html +++ b/docs/reference/is.neuronlist.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/is.nrrd.html b/docs/reference/is.nrrd.html index 1647e048..1eed3055 100644 --- a/docs/reference/is.nrrd.html +++ b/docs/reference/is.nrrd.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/is.swc.html b/docs/reference/is.swc.html index 1ef4acae..2f262e76 100644 --- a/docs/reference/is.swc.html +++ b/docs/reference/is.swc.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/is.vaa3draw.html b/docs/reference/is.vaa3draw.html index c472a687..f0c9649a 100644 --- a/docs/reference/is.vaa3draw.html +++ b/docs/reference/is.vaa3draw.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git 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a/docs/reference/neuronlist-dataframe-methods.html +++ b/docs/reference/neuronlist-dataframe-methods.html @@ -74,7 +74,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/neuronlist.html b/docs/reference/neuronlist.html index 2df44b1b..52526d9c 100644 --- a/docs/reference/neuronlist.html +++ b/docs/reference/neuronlist.html @@ -72,7 +72,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/neuronlistfh.html b/docs/reference/neuronlistfh.html index 840d7e1a..88cb993f 100644 --- a/docs/reference/neuronlistfh.html +++ b/docs/reference/neuronlistfh.html @@ -79,7 +79,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/ngraph.html b/docs/reference/ngraph.html index d904a8c6..4fd1a247 100644 --- a/docs/reference/ngraph.html +++ b/docs/reference/ngraph.html @@ -71,7 +71,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/nlapply.html b/docs/reference/nlapply.html index f0a42f6f..162d5d1c 100644 --- a/docs/reference/nlapply.html +++ b/docs/reference/nlapply.html @@ -67,7 +67,7 @@ nat - 1.9.0 + 1.9.1 @@ -278,7 +278,7 @@

    Examp ## plyr+parallel library(doMC) # can also specify cores e.g. registerDoMC(cores=4) -registerDoMC() +registerDoMC() system.time(d2<-dotprops(kcs20,resample=1,k=5,.parallel=TRUE)) stopifnot(all.equal(d1,d2)) # }
    diff --git a/docs/reference/nlscan.html b/docs/reference/nlscan.html index 687f6b95..bbee839d 100644 --- a/docs/reference/nlscan.html +++ b/docs/reference/nlscan.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1
    diff --git a/docs/reference/nopen3d.html b/docs/reference/nopen3d.html index bfe5e87b..49925f55 100644 --- a/docs/reference/nopen3d.html +++ b/docs/reference/nopen3d.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/normalise_swc.html b/docs/reference/normalise_swc.html index c8657059..e4bcab55 100644 --- a/docs/reference/normalise_swc.html +++ b/docs/reference/normalise_swc.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/npop3d.html b/docs/reference/npop3d.html index db28057b..a2ccb646 100644 --- a/docs/reference/npop3d.html +++ b/docs/reference/npop3d.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/nrrd.voxdims.html b/docs/reference/nrrd.voxdims.html index 3dddb0b3..6986bc52 100644 --- a/docs/reference/nrrd.voxdims.html +++ b/docs/reference/nrrd.voxdims.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/nvertices.html b/docs/reference/nvertices.html index b5447c06..e751f460 100644 --- a/docs/reference/nvertices.html +++ b/docs/reference/nvertices.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/nview3d.html b/docs/reference/nview3d.html index e7db817e..5121a888 100644 --- a/docs/reference/nview3d.html +++ b/docs/reference/nview3d.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/origin.html b/docs/reference/origin.html index c1fb4446..8c9e6258 100644 --- a/docs/reference/origin.html +++ b/docs/reference/origin.html @@ -65,7 +65,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/pan3d.html b/docs/reference/pan3d.html index ad3a6f27..ffb11fbb 100644 --- a/docs/reference/pan3d.html +++ b/docs/reference/pan3d.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/plane_coefficients.html b/docs/reference/plane_coefficients.html index 4b661bb4..b0683b5e 100644 --- a/docs/reference/plane_coefficients.html +++ b/docs/reference/plane_coefficients.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/plot.neuron.html b/docs/reference/plot.neuron.html index 6030107c..ffb44d7f 100644 --- a/docs/reference/plot.neuron.html +++ b/docs/reference/plot.neuron.html @@ -65,7 +65,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/plot.neuronlist.html b/docs/reference/plot.neuronlist.html index 8cddda8e..49bcf8d4 100644 --- a/docs/reference/plot.neuronlist.html +++ b/docs/reference/plot.neuronlist.html @@ -66,7 +66,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/plot3d.boundingbox.html b/docs/reference/plot3d.boundingbox.html index 22cb88a3..cc735a12 100644 --- a/docs/reference/plot3d.boundingbox.html +++ b/docs/reference/plot3d.boundingbox.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/plot3d.cmtkreg.html b/docs/reference/plot3d.cmtkreg.html index d81b83e2..6c3628c6 100644 --- a/docs/reference/plot3d.cmtkreg.html +++ b/docs/reference/plot3d.cmtkreg.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 @@ -164,18 +164,16 @@

    See a

    Examples

    -
    # NOT RUN {
    -testdatadir=system.file("tests/testthat/testdata/cmtk", package="nat")
    +    
    testdatadir=system.file("tests/testthat/testdata/cmtk", package="nat") regpath=file.path(testdatadir,'FCWB_JFRC2_01_warp_level-01.list/') +# only run this if file is present (not always installed) +if(file.exists(regpath)){ plot3d(cmtkreg(regpath)) # or read registration into memory if you want to work with it reg=read.cmtkreg(regpath) -# nb calling plot3d.cmtkreg directly (rather than using the generic plot3d) -# is considered bad style but read.cmtkreg returns a plain list -# so method dispatch will fail -nat:::plot3d.cmtkreg(reg) -# }
    +plot3d(reg) +}
    diff --git a/docs/reference/plot3d.html b/docs/reference/plot3d.html index 77e98af4..12df5cb6 100644 --- a/docs/reference/plot3d.html +++ b/docs/reference/plot3d.html @@ -65,7 +65,7 @@ nat - 1.9.0 + 1.9.1 @@ -155,12 +155,13 @@

    See a

    Examples

    # all known plot3d methods -methods("plot3d")
    #> [1] plot3d.boundingbox* plot3d.character* plot3d.cmtkreg* -#> [4] plot3d.default* plot3d.deldir* plot3d.dotprops* -#> [7] plot3d.function* plot3d.hxsurf* plot3d.mesh3d* -#> [10] plot3d.neuron* plot3d.neuronlist* plot3d.rglbackground* -#> [13] plot3d.rglbboxdeco* plot3d.rglobject* plot3d.rglscene* -#> [16] plot3d.rglsubscene* +methods("plot3d")
    #> [1] plot3d.ashape3d* plot3d.boundingbox* plot3d.character* +#> [4] plot3d.cmtkreg* plot3d.default* plot3d.deldir* +#> [7] plot3d.dotprops* plot3d.formula* plot3d.function* +#> [10] plot3d.hxsurf* plot3d.lm* plot3d.mesh3d* +#> [13] plot3d.neuron* plot3d.neuronlist* plot3d.rglbackground* +#> [16] plot3d.rglbboxdeco* plot3d.rglobject* plot3d.rglscene* +#> [19] plot3d.rglsubscene* plot3d.tri* plot3d.triSht* #> see '?methods' for accessing help and source code
    # up to date list of all plot3d nethods in this package intersect(methods("plot3d"), ls(asNamespace("nat")))
    #> [1] "plot3d.boundingbox" "plot3d.character" "plot3d.cmtkreg" diff --git a/docs/reference/plot3d.hxsurf.html b/docs/reference/plot3d.hxsurf.html index cb307406..818adeba 100644 --- a/docs/reference/plot3d.hxsurf.html +++ b/docs/reference/plot3d.hxsurf.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1
    diff --git a/docs/reference/plot3d.neuron.html b/docs/reference/plot3d.neuron.html index 8ff16e5e..5f5a37a2 100644 --- a/docs/reference/plot3d.neuron.html +++ b/docs/reference/plot3d.neuron.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/plot3d.neuronlist.html b/docs/reference/plot3d.neuronlist.html index 6f082c87..5562413b 100644 --- a/docs/reference/plot3d.neuronlist.html +++ b/docs/reference/plot3d.neuronlist.html @@ -66,7 +66,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/pointsinside.html b/docs/reference/pointsinside.html index 3f751cc1..ce992fe9 100644 --- a/docs/reference/pointsinside.html +++ b/docs/reference/pointsinside.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/potential_synapses.html b/docs/reference/potential_synapses.html index 64cfe307..5188d9e9 100644 --- a/docs/reference/potential_synapses.html +++ b/docs/reference/potential_synapses.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/projection.html b/docs/reference/projection.html index c2f6b708..487ccb72 100644 --- a/docs/reference/projection.html +++ b/docs/reference/projection.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/prune.html b/docs/reference/prune.html index 54729007..0052ff16 100644 --- a/docs/reference/prune.html +++ b/docs/reference/prune.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/prune_strahler.html b/docs/reference/prune_strahler.html index 25ff104e..7ffad6f1 100644 --- a/docs/reference/prune_strahler.html +++ b/docs/reference/prune_strahler.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/prune_vertices.html b/docs/reference/prune_vertices.html index 9597bf86..930d86e5 100644 --- a/docs/reference/prune_vertices.html +++ b/docs/reference/prune_vertices.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/read.cmtk.html b/docs/reference/read.cmtk.html index 2501d4c5..4b8a1adc 100644 --- a/docs/reference/read.cmtk.html +++ b/docs/reference/read.cmtk.html @@ -65,7 +65,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/read.cmtkreg.html b/docs/reference/read.cmtkreg.html index b876786f..341e4dcc 100644 --- a/docs/reference/read.cmtkreg.html +++ b/docs/reference/read.cmtkreg.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/read.hxsurf.html b/docs/reference/read.hxsurf.html index 5e485e97..404403db 100644 --- a/docs/reference/read.hxsurf.html +++ b/docs/reference/read.hxsurf.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/read.landmarks.html b/docs/reference/read.landmarks.html index 775dee85..9ac56b21 100644 --- a/docs/reference/read.landmarks.html +++ b/docs/reference/read.landmarks.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 @@ -233,16 +233,16 @@

    Examp m=matrix(rnorm(6), ncol=3) rownames(m)=c("nose", "ear") f=write.landmarks(m, file='knee', format='cmtk') -read.landmarks(f)
    #> [,1] [,2] [,3] -#> nose 0.1766739 1.7054692 0.5559072 -#> ear -0.7190785 -0.2678327 -0.5169957 +read.landmarks(f)
    #> [,1] [,2] [,3] +#> nose 0.1776791 0.4336128 0.8724661 +#> ear 0.3655107 -1.5143780 0.1380608 #> attr(,"class") #> [1] "landmarks" "matrix"
    # write in amira format which does not support named landmarks f2=write.landmarks(m, file='knee', format='amira') -read.landmarks(f2)
    #> [,1] [,2] [,3] -#> [1,] 0.1766739 1.7054692 0.5559072 -#> [2,] -0.7190785 -0.2678327 -0.5169957 +read.landmarks(f2)
    #> [,1] [,2] [,3] +#> [1,] 0.1776791 0.4336128 0.8724661 +#> [2,] 0.3655107 -1.5143780 0.1380608 #> attr(,"class") #> [1] "landmarks" "matrix"
    # clean up diff --git a/docs/reference/read.morphml.html b/docs/reference/read.morphml.html index d30c532d..a2d70d46 100644 --- a/docs/reference/read.morphml.html +++ b/docs/reference/read.morphml.html @@ -67,7 +67,7 @@ nat - 1.9.0 + 1.9.1
    diff --git a/docs/reference/read.neuron.fiji.html b/docs/reference/read.neuron.fiji.html index c6aadaf5..b25fbc76 100644 --- a/docs/reference/read.neuron.fiji.html +++ b/docs/reference/read.neuron.fiji.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/read.neuron.html b/docs/reference/read.neuron.html index 58f9a103..2ede7427 100644 --- a/docs/reference/read.neuron.html +++ b/docs/reference/read.neuron.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/read.neuron.neuroml.html b/docs/reference/read.neuron.neuroml.html index a0b3f266..0535f092 100644 --- a/docs/reference/read.neuron.neuroml.html +++ b/docs/reference/read.neuron.neuroml.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/read.neuron.swc.html b/docs/reference/read.neuron.swc.html index f211bbdd..4eed5b90 100644 --- a/docs/reference/read.neuron.swc.html +++ b/docs/reference/read.neuron.swc.html @@ -67,7 +67,7 @@ nat - 1.9.0 + 1.9.1 @@ -188,7 +188,7 @@

    Details

    SWC Format

    According to - http://research.mssm.edu/cnic/swc.html SWC file format has a + http://www.neuronland.org/NLMorphologyConverter/MorphologyFormats/SWC/Spec.html SWC file format has a radius not a diameter specification

    See also

    diff --git a/docs/reference/read.neuronlistfh.html b/docs/reference/read.neuronlistfh.html index cee32d98..1a66202e 100644 --- a/docs/reference/read.neuronlistfh.html +++ b/docs/reference/read.neuronlistfh.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/read.neurons.html b/docs/reference/read.neurons.html index 7948032b..430ab39f 100644 --- a/docs/reference/read.neurons.html +++ b/docs/reference/read.neurons.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 @@ -245,7 +245,7 @@

    Examp # fetch table of worm neurons from wormbase library(rvest) nlurl="http://wormatlas.org/neurons/Individual%20Neurons/Neuronframeset.html" -wormneurons = html_table(read_html(nlurl), fill=TRUE)[[4]] +wormneurons = html_table(read_html(nlurl), fill=TRUE)[[4]] vddf=subset(wormneurons, Neuron%in%vds) rownames(vddf)=vddf$Neuron # attach metadata to neuronlist diff --git a/docs/reference/read.nrrd.html b/docs/reference/read.nrrd.html index f748cd25..237a661f 100644 --- a/docs/reference/read.nrrd.html +++ b/docs/reference/read.nrrd.html @@ -68,7 +68,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/read.vaa3draw.html b/docs/reference/read.vaa3draw.html index 350fdb48..a28ebaf1 100644 --- a/docs/reference/read.vaa3draw.html +++ b/docs/reference/read.vaa3draw.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/reglist.html b/docs/reference/reglist.html index ce22736c..3af8959e 100644 --- a/docs/reference/reglist.html +++ b/docs/reference/reglist.html @@ -79,7 +79,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/remotesync.html b/docs/reference/remotesync.html index 46690fdd..5bbbaa99 100644 --- a/docs/reference/remotesync.html +++ b/docs/reference/remotesync.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/resample.html b/docs/reference/resample.html index a13727a4..e103f340 100644 --- a/docs/reference/resample.html +++ b/docs/reference/resample.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/rootpoints.html b/docs/reference/rootpoints.html index cfb0d71e..c18bea3c 100644 --- a/docs/reference/rootpoints.html +++ b/docs/reference/rootpoints.html @@ -67,7 +67,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/scale.neuron.html b/docs/reference/scale.neuron.html index eeefa538..54ee35c1 100644 --- a/docs/reference/scale.neuron.html +++ b/docs/reference/scale.neuron.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/seglengths.html b/docs/reference/seglengths.html index 50d37cc5..c97c5139 100644 --- a/docs/reference/seglengths.html +++ b/docs/reference/seglengths.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/seglist.html b/docs/reference/seglist.html index 563bb3c3..e185cdbb 100644 --- a/docs/reference/seglist.html +++ b/docs/reference/seglist.html @@ -73,7 +73,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/seglist2swc.html b/docs/reference/seglist2swc.html index a746c7fb..865aa9fd 100644 --- a/docs/reference/seglist2swc.html +++ b/docs/reference/seglist2swc.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/segmentgraph-1.png b/docs/reference/segmentgraph-1.png index 0606cd5cc7e78eaed108cc155a1d1068f2f0ace4..cf9372a7f925b05cc974da164e805d3910aee1d4 100644 GIT binary patch literal 86192 zcmeEu`9IYA7k^QyXmN!k6gNUl$i9q{RJLSar-(_G2{G20N$5&qjW9g&QwI!Y=Wv>f^%gN z(UBZTO`)U)kSoBHgHsi`7IAM*?dM4>km8jc^$Lv+QDGMe=O z|MH&>{dHa%ut;a8@k^uKVFyi(ABDxAjBamOyTL)1`O*hU*MBsdJ4O0vpX5zQFM3S5 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+198,43 @@

    Examp #> $ :List of 1 #> ..$ : 'igraph.vs' int 2 #> .. ..- attr(*, "env")=<weakref> -#> .. ..- attr(*, "graph")= chr "90309481-e6a2-45d7-a916-d2341a57edf2" +#> .. ..- attr(*, "graph")= chr "cdbd10ba-ccbd-44c6-b3ce-53cdfa1f1f26" #> $ :List of 1 #> ..$ : 'igraph.vs' int [1:2] 3 4 #> .. ..- attr(*, "env")=<weakref> -#> .. ..- attr(*, "graph")= chr "90309481-e6a2-45d7-a916-d2341a57edf2" +#> .. ..- attr(*, "graph")= chr "cdbd10ba-ccbd-44c6-b3ce-53cdfa1f1f26" #> $ :List of 1 #> ..$ : 'igraph.vs' int(0) #> .. ..- attr(*, "env")=<weakref> -#> .. ..- attr(*, "graph")= chr "90309481-e6a2-45d7-a916-d2341a57edf2" +#> .. ..- attr(*, "graph")= chr "cdbd10ba-ccbd-44c6-b3ce-53cdfa1f1f26" #> $ :List of 1 #> ..$ : 'igraph.vs' int [1:2] 5 8 #> .. ..- attr(*, "env")=<weakref> -#> .. ..- attr(*, "graph")= chr "90309481-e6a2-45d7-a916-d2341a57edf2" +#> .. ..- attr(*, "graph")= chr "cdbd10ba-ccbd-44c6-b3ce-53cdfa1f1f26" #> $ :List of 1 #> ..$ : 'igraph.vs' int [1:2] 6 7 #> .. ..- attr(*, "env")=<weakref> -#> .. ..- attr(*, "graph")= chr "90309481-e6a2-45d7-a916-d2341a57edf2" +#> .. ..- attr(*, "graph")= chr "cdbd10ba-ccbd-44c6-b3ce-53cdfa1f1f26" #> $ :List of 1 #> ..$ : 'igraph.vs' int(0) #> .. ..- attr(*, "env")=<weakref> -#> .. ..- attr(*, "graph")= chr "90309481-e6a2-45d7-a916-d2341a57edf2" +#> .. ..- attr(*, "graph")= chr "cdbd10ba-ccbd-44c6-b3ce-53cdfa1f1f26" #> $ :List of 1 #> ..$ : 'igraph.vs' int(0) #> .. ..- attr(*, "env")=<weakref> -#> .. ..- attr(*, "graph")= chr "90309481-e6a2-45d7-a916-d2341a57edf2" +#> .. ..- attr(*, "graph")= chr "cdbd10ba-ccbd-44c6-b3ce-53cdfa1f1f26" #> $ :List of 1 #> ..$ : 'igraph.vs' int [1:2] 9 12 #> .. ..- attr(*, "env")=<weakref> -#> .. ..- attr(*, "graph")= chr "90309481-e6a2-45d7-a916-d2341a57edf2" +#> .. ..- attr(*, "graph")= chr "cdbd10ba-ccbd-44c6-b3ce-53cdfa1f1f26" #> $ :List of 1 #> ..$ : 'igraph.vs' int [1:2] 10 11 #> .. ..- attr(*, "env")=<weakref> -#> .. ..- attr(*, "graph")= chr "90309481-e6a2-45d7-a916-d2341a57edf2" +#> .. ..- attr(*, "graph")= chr "cdbd10ba-ccbd-44c6-b3ce-53cdfa1f1f26" #> $ :List of 1 #> ..$ : 'igraph.vs' int(0) #> .. ..- attr(*, "env")=<weakref> -#> .. ..- attr(*, "graph")= chr "90309481-e6a2-45d7-a916-d2341a57edf2" +#> .. ..- attr(*, "graph")= chr "cdbd10ba-ccbd-44c6-b3ce-53cdfa1f1f26" #> - attr(*, "class")= chr "igraph"
    library(igraph) plot(sg, edge.arrow.size=.4, vertex.size=10)

    diff --git a/docs/reference/setdiff.html b/docs/reference/setdiff.html index 68dab3ef..600f8aeb 100644 --- a/docs/reference/setdiff.html +++ b/docs/reference/setdiff.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/simplify_reglist.html b/docs/reference/simplify_reglist.html index 28cc8760..8c834b34 100644 --- a/docs/reference/simplify_reglist.html +++ b/docs/reference/simplify_reglist.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/smooth_neuron.html b/docs/reference/smooth_neuron.html index a9e219dc..a6e27be6 100644 --- a/docs/reference/smooth_neuron.html +++ b/docs/reference/smooth_neuron.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/spine.html b/docs/reference/spine.html index 539367ab..fdf5d564 100644 --- a/docs/reference/spine.html +++ b/docs/reference/spine.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/strahler_order.html b/docs/reference/strahler_order.html index 65f03a6c..27aa48d7 100644 --- a/docs/reference/strahler_order.html +++ b/docs/reference/strahler_order.html @@ -66,7 +66,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/sub-.neuronlistfh.html b/docs/reference/sub-.neuronlistfh.html index a4df89ca..b27d9d80 100644 --- a/docs/reference/sub-.neuronlistfh.html +++ b/docs/reference/sub-.neuronlistfh.html @@ -65,7 +65,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/sub2ind.html b/docs/reference/sub2ind.html index a95d1652..c9557f82 100644 --- a/docs/reference/sub2ind.html +++ b/docs/reference/sub2ind.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/subset.dotprops.html b/docs/reference/subset.dotprops.html index d72cc1a3..b838219f 100644 --- a/docs/reference/subset.dotprops.html +++ b/docs/reference/subset.dotprops.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/subset.html b/docs/reference/subset.html index ab59bdb4..749d407c 100644 --- a/docs/reference/subset.html +++ b/docs/reference/subset.html @@ -65,7 +65,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/subset.hxsurf.html b/docs/reference/subset.hxsurf.html index 66653dac..bf7e1578 100644 --- a/docs/reference/subset.hxsurf.html +++ b/docs/reference/subset.hxsurf.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/subset.neuron.html b/docs/reference/subset.neuron.html index ec79e7ac..2d3cee0f 100644 --- a/docs/reference/subset.neuron.html +++ b/docs/reference/subset.neuron.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/subset.neuronlist.html b/docs/reference/subset.neuronlist.html index 67a210ec..3d9b6069 100644 --- a/docs/reference/subset.neuronlist.html +++ b/docs/reference/subset.neuronlist.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/summary.neuron.html b/docs/reference/summary.neuron.html index ccb0917a..2871f75a 100644 --- a/docs/reference/summary.neuron.html +++ b/docs/reference/summary.neuron.html @@ -67,7 +67,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/threshold.html b/docs/reference/threshold.html index 2f5cbde7..174b2492 100644 --- a/docs/reference/threshold.html +++ b/docs/reference/threshold.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/union.html b/docs/reference/union.html index c41b9bc8..65f98490 100644 --- a/docs/reference/union.html +++ b/docs/reference/union.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/unmask.html b/docs/reference/unmask.html index 7b5cb3bb..3c8ed369 100644 --- a/docs/reference/unmask.html +++ b/docs/reference/unmask.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/voxdims.html b/docs/reference/voxdims.html index 1e3af5fd..ebea7859 100644 --- a/docs/reference/voxdims.html +++ b/docs/reference/voxdims.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/write.amiramesh.html b/docs/reference/write.amiramesh.html index f4d2d0db..b9377919 100644 --- a/docs/reference/write.amiramesh.html +++ b/docs/reference/write.amiramesh.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/write.cmtk.html b/docs/reference/write.cmtk.html index 317692a5..22105dae 100644 --- a/docs/reference/write.cmtk.html +++ b/docs/reference/write.cmtk.html @@ -65,7 +65,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/write.cmtkreg.html b/docs/reference/write.cmtkreg.html index 85508619..617a77b7 100644 --- a/docs/reference/write.cmtkreg.html +++ b/docs/reference/write.cmtkreg.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/write.hxsurf.html b/docs/reference/write.hxsurf.html index eb01f267..428e5247 100644 --- a/docs/reference/write.hxsurf.html +++ b/docs/reference/write.hxsurf.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/write.neuron.html b/docs/reference/write.neuron.html index 40974ede..c2f7e1f9 100644 --- a/docs/reference/write.neuron.html +++ b/docs/reference/write.neuron.html @@ -67,7 +67,7 @@ nat - 1.9.0 + 1.9.1 @@ -169,7 +169,8 @@

    Arg format

    Unique abbreviation of one of the registered file formats for -neurons including 'swc', 'hxlineset', 'hxskel'

    +neurons including 'swc', 'hxlineset', 'hxskel', if no format can be extracted from +the filename and the ext parameter, then it defaults to 'swc'

    ext diff --git a/docs/reference/write.neuronlistfh.html b/docs/reference/write.neuronlistfh.html index 587fd03a..6c22ed4a 100644 --- a/docs/reference/write.neuronlistfh.html +++ b/docs/reference/write.neuronlistfh.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/write.neurons.html b/docs/reference/write.neurons.html index f61f8b50..6345fc75 100644 --- a/docs/reference/write.neurons.html +++ b/docs/reference/write.neurons.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 @@ -157,7 +157,8 @@

    Arg format

    Unique abbreviation of one of the registered file formats for -neurons including 'swc', 'hxlineset', 'hxskel'

    +neurons including 'swc', 'hxlineset', 'hxskel', if no format can be extracted from +the filename and the ext parameter, then it defaults to 'swc'

    subdir diff --git a/docs/reference/write.nrrd.html b/docs/reference/write.nrrd.html index d236d05a..c400b7d5 100644 --- a/docs/reference/write.nrrd.html +++ b/docs/reference/write.nrrd.html @@ -71,7 +71,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/write.vtk.html b/docs/reference/write.vtk.html index d031b09e..b2267834 100644 --- a/docs/reference/write.vtk.html +++ b/docs/reference/write.vtk.html @@ -63,7 +63,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/xform.html b/docs/reference/xform.html index 08fddf2d..067223c3 100644 --- a/docs/reference/xform.html +++ b/docs/reference/xform.html @@ -71,7 +71,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/xformimage.html b/docs/reference/xformimage.html index 429951dd..31ccb2c7 100644 --- a/docs/reference/xformimage.html +++ b/docs/reference/xformimage.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/xformpoints.html b/docs/reference/xformpoints.html index 4c053b50..e3657842 100644 --- a/docs/reference/xformpoints.html +++ b/docs/reference/xformpoints.html @@ -64,7 +64,7 @@ nat - 1.9.0 + 1.9.1 diff --git a/docs/reference/xyzmatrix.html b/docs/reference/xyzmatrix.html index de8edf14..2e2814d9 100644 --- a/docs/reference/xyzmatrix.html +++ b/docs/reference/xyzmatrix.html @@ -66,7 +66,7 @@ nat - 1.9.0 + 1.9.1