Why are the poscon concentrations different across plate types?

[shntnu]

From an experimental point of view, why did you put the compounds at 3.75uM in the MoA plates, at 5uM in the target plates (and the positive controls), when the rest of the compounds in the dataset are at 10uM? Is it because, given that they were selected for their labelled profiles, they are sufficiently labelled at 5uM (and would potentially be too toxic at 10uM)?

[AnneCarpenter]

Compounds in the MOA and Target1 and Target 2 sets were selected from compounds in the Drug Repurposing Hub, meaning that they have been optimized for some biological function and likely have fairly small EC50/IC50s. For this reason we used them at lower than 10 uM (which is a typical screening concentration for libraries of unoptimized compounds.

An additional clarification: none of those compounds (except the small number of positive controls) were selected for having a known Cell Painting phenotype. They were selected based on having annotations for particular MOAs and some other criteria unrelated to an imaging phenotype.