From e503bf607aac7a81ace16dcb0ff150f9500b67d3 Mon Sep 17 00:00:00 2001
From: tfenne <tfenne@tfenne.com>
Date: Fri, 15 Nov 2024 14:39:20 -0700
Subject: [PATCH] Add a comment

---
 prymer/api/picking.py | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/prymer/api/picking.py b/prymer/api/picking.py
index 1315881..796628e 100644
--- a/prymer/api/picking.py
+++ b/prymer/api/picking.py
@@ -146,6 +146,8 @@ def build_primer_pairs(
                 if amp_span.length > amplicon_sizes.max:
                     continue
 
+                # Since the amplicon span and the region_start are both 1-based, the minuend
+                # becomes a zero-based offset
                 amp_bases = bases[amp_span.start - region_start : amp_span.end - region_start + 1]
                 amp_tm = calculate_long_seq_tm(amp_bases)