From 7ae349c7f642cb5699627d7ebe7e76423cf8a642 Mon Sep 17 00:00:00 2001
From: Erin McAuley <erin@fulcrumgenomics.com>
Date: Thu, 3 Oct 2024 14:25:43 -0400
Subject: [PATCH] style: add additional details on thermo defaults

---
 prymer/primer3/primer3_parameters.py | 5 ++++-
 1 file changed, 4 insertions(+), 1 deletion(-)

diff --git a/prymer/primer3/primer3_parameters.py b/prymer/primer3/primer3_parameters.py
index 282313c..e8a01a0 100644
--- a/prymer/primer3/primer3_parameters.py
+++ b/prymer/primer3/primer3_parameters.py
@@ -195,7 +195,10 @@ class ProbeParameters:
     If they are not provided, `probe_max_self_any_thermo`, `probe_max_self_end_thermo`, and
     `probe_max_hairpin_thermo` will be set to default values as specified in the Primer3 manual.
     The default value is 10 degrees lower than the minimal melting temperature specified for
-    probe design.
+    probe design (i.e., when not provided, values are specified relative to the probe design).
+    If these values are provided, users should provide the absolute value of the
+    melting temperature threshold (i.e., when provided, values should be specified as independent
+    of probe design.)
 
     """