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Run only bin evaluations #46

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adityabandla opened this issue Sep 24, 2019 · 1 comment
Open

Run only bin evaluations #46

adityabandla opened this issue Sep 24, 2019 · 1 comment

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@adityabandla
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Is it possible to run DAS tool in a modular fashion? For a set of genomes, I dereplicated them using dRep and would now like to assess their completeness & redundancy. I prefer using the SCGs used by DAS tool and I don't think there is straight forward way of using this set with CheckM

@cmks
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cmks commented Oct 4, 2019

Unfortunately, it's not possible to run DAS Tool in a modular way yet. However, if you run DAS Tool with only one input bin-set, it'll give you what you're looking for. For this hack, you need a contigs2bin table of your dRep bins and all contigs stored in one fasta file (if you have one fasta file per bin you can use the Fasta_to_Scaffolds2Bin.sh script from the src dir of this repo).
After running DAS Tool, the completeness prediction will be in the .eval file.

For example using the sample_data this would look like:

DAS_Tool -i sample.human.gut_maxbin2_scaffolds2bin.tsv -c sample.human.gut_contigs.fa -o evaltest -l dRep

The SCG results are in evaltest_dRep.eval.

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