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Describe the bug
When running demultiplexing of MTC 16S Run23, one sample, "EAR4", ended up with one more read in the reverse file than in the forward after the strip_error_barcodes step. I used diff, zgrep, and awk to determine the extraneous sample and removed it. This was that read:
Completely unclear to me why this has happened, never happened before. Will have to take a closer look at this read, its associated forward read. I suspect it has something to do with a weird special character.
The text was updated successfully, but these errors were encountered:
Describe the bug
When running demultiplexing of MTC 16S Run23, one sample, "EAR4", ended up with one more read in the reverse file than in the forward after the strip_error_barcodes step. I used diff, zgrep, and awk to determine the extraneous sample and removed it. This was that read:
@M02617:814:GW231120000:1:1114:21145:14315 2:N:0:CGTAGCGA-CTACTATA GCCAGTTTGGGTCTTGGCTATTGTGAGATCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGTTTTTTACAACTCAGGTGAGTTTTAGCTTTATTGGGGAGGGTGTGATCTAAAACACTCTTTACGCCGGCTTCTA
+
111>A113@111A1B1B1BDBAG333D3311FF3GF2D2AF2FBECGB11F?GEFFEF2DDDHHFGFHDGHF00F01A12//BFHHFGHHFEFGHAGHHGFGHHHHGFHGFH?FGGG/?E/GFGBGDF1BGGGFHHHHEFC?BC/BCCGF
Completely unclear to me why this has happened, never happened before. Will have to take a closer look at this read, its associated forward read. I suspect it has something to do with a weird special character.
The text was updated successfully, but these errors were encountered: