Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Nonstandard dual-barcode lengths break pheniqs #462

Open
adamcantor22 opened this issue Dec 8, 2023 · 0 comments
Open

Nonstandard dual-barcode lengths break pheniqs #462

adamcantor22 opened this issue Dec 8, 2023 · 0 comments

Comments

@adamcantor22
Copy link
Member

Describe the bug
Pheniqs configuration is currently hardcoded to only support forward and reverse barcodes of 8bp each. This is by far the most common situation, but I ran into a situation in which the forward barcodes were 12bp (I believe this used to be more common). This caused pheniqs to break, and I had to go in manually and fix it.

To Reproduce
Demultiplex a run using pheniqs with either barcode length not being 8bp

Solution
Configure pheniqs to automatically take whatever length of barcodes is present in the metadata for each run. This will not affect the error case of two barcodes in the same run/direction being different lengths, that will still error on upload.

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Projects
None yet
Development

No branches or pull requests

1 participant