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<!DOCTYPE HTML>
<html lang="en"><head><meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
<title>John Stout</title>
<meta name="author" content="John Stout">
<meta name="viewport" content="width=device-width, initial-scale=1">
<link rel="stylesheet" type="text/css" href="stylesheet.css">
<link rel="icon" href="data:image/svg+xml,<svg xmlns=%22http://www.w3.org/2000/svg%22 viewBox=%220 0 100 100%22><text y=%22.9em%22 font-size=%2290%22>🌐</text></svg>">
</head>
<body>
<table style="width:100%;max-width:800px;border:0px;border-spacing:0px;border-collapse:separate;margin-right:auto;margin-left:auto;"><tbody>
<tr style="padding:0px">
<td style="padding:0px">
<table style="width:100%;border:0px;border-spacing:0px;border-collapse:separate;margin-right:auto;margin-left:auto;"><tbody>
<tr style="padding:0px">
<td style="padding:2.5%;width:63%;vertical-align:middle">
<p style="text-align:center">
<name>John Stout</name>
</p>
<p>I am a doctoral student in neuroscience at the University of Delaware where I study the mechanisms supporting learning and memory.
</p>
<p style="text-align:center">
<a href="mailto:[email protected]">Email</a>  / 
<a href="data/John Stout CV - 2022.pdf">CV</a>  / 
<a href="data/JonBarron-bio.txt">Bio</a>  / 
<a href="https://scholar.google.com/citations?user=va49pf0AAAAJ&hl=en&oi=ao">Google Scholar</a>  / 
<a href="https://twitter.com/_johnStout_">Twitter</a>  / 
<a href="https://github.com/JohnStout">Github</a>  / 
<a href="https://github.com/GriffinLabCode">MATLAB neuroscience pipeline that I developed for my advisors laboratory</a>
</p>
</td>
<td style="padding:2.5%;width:40%;max-width:40%">
<a href="data/AlexAndMe.jpg"><img style="width:100%;max-width:100%" alt="profile photo" src="data/AlexAndMe.jpg" class="hoverZoomLink"></a>
</td>
</tr>
</tbody></table>
<table style="width:100%;border:0px;border-spacing:0px;border-collapse:separate;margin-right:auto;margin-left:auto;"><tbody>
<tr>
<td style="padding:20px;width:100%;vertical-align:middle">
<heading>Research</heading>
<p>
My interests are centered around understanding 1) the neural mechanisms supporting memory and executive functioning, 2) how these processes are disrupted with disorders of the brain, and 3) in the development of novel techniques to improve cognition.
</p>
<p>
To answer these questions, I have acquired skills in electrophysiology, optogenetics, drug-infusion procedures, brain machine interfacing approaches, and data analysis. My research goal is to combine anatomical, molecular, and circuit manipulations to further address these questions. In terms of long term goals, I hope that my research will one day provide translational insights to improve the lives of those suffering from neuropsychiatric and neurodevelopmental disorders.
</p>
</td>
</tr>
</table>
<table style="width:100%;border:0px;border-spacing:0px;border-collapse:separate;margin-right:auto;margin-left:auto;"><tbody>
<tr onmouseout="malle_stop()" onmouseover="malle_start()">
<td style="padding:40px;width:25%;vertical-align:middle">
<div class="one">
<div class="two" id='fig1'>
<img src='data/fig_VTE_Thalamus.png' width="200"></div>
<img src='data/fig_VTE_Thalamus.png' width="200">
</div>
</td>
<td style="padding:40px;width:75%;vertical-align:middle">
<a href="https://www.nature.com/articles/s41598-022-14707-8">
<papertitle>The ventral midline thalamus coordinates prefrontal–hippocampal neural synchrony during vicarious trial and error</papertitle>
</a>
<br>
<strong> John Stout </strong>,
<a href="https://scholar.google.com/citations?user=1jsGGfoAAAAJ&hl=en&oi=ao">Henry Hallock</a>,
Allison George, Suhaas Adiraju,
<a href="https://scholar.google.com/citations?user=b_sVYeUAAAAJ&hl=en&oi=ao">Amy Griffin</a>
<em>Scientific Reports</em>, 2022
<br>
<a href="https://www.nature.com/articles/s41598-022-14707-8">Paper</a>
/
<p>
Deliberation, the process where we contemplate options before a choice is made, is thought to occur when rats pause and reorient during decision-points on mazes. Using pharmacological inactivation procedures, we tested whether a thalamic region connecting prefrontal and hippocampal regions was implicated in deliberation. We found that thalamic inactivation disrupted deliberation along with prefrontal-hippocampal neural interactions.
</p>
</td>
</tr>
<tr onmouseout="malle_stop()" onmouseover="malle_start()">
<td style="padding:40px;width:25%;vertical-align:middle">
<div class="one">
<div class="two" id='malle_image'>
<img src='data/Fig_Stout2020.png' width="220"></div>
<img src='data/Fig_Stout2020.png' width="220">
</div>
</td>
<td style="padding:40px;width:75%;vertical-align:middle">
<a href="https://www.frontiersin.org/articles/10.3389/fnbeh.2020.00151/full">
<papertitle>Representations of On-Going Behavior and Future Actions During a Spatial Working Memory Task by a High Firing-Rate Population of Medial Prefrontal Cortex Neurons.</papertitle>
</a>
<br>
<strong> John Stout </strong>, and
<a href="https://scholar.google.com/citations?user=b_sVYeUAAAAJ&hl=en&oi=ao">Amy Griffin</a>
<em>Frontiers in Behavioral Neuroscience</em>, 2022
<br>
<a href="https://www.frontiersin.org/articles/10.3389/fnbeh.2020.00151/full">Paper</a>
/
<p>
To better understand the involvement of the prefrontal cortex in memory-guided decision making, we recorded action potentials from the medial prefrontal cortex as rats engaged in a working memory task that experimentally dissociates encoding-dominant processes from decision processes. Using machine learning and modeling procedures, we discovered that the prefrontal cortex predominately represents actions. Both sparsely active and highly active neurons represent current actions, but only highly active neurons engage in representations of future outcomes. Interestingly, a greater number of neurons were required to predict a future action than a current action, consistent with increased cognitive load. This study provided a better mechanistic understanding of how certain prefrontal cortex populations are engaged in executive functioning.
</p>
</td>
</tr>
</tbody></table>
<table style="width:100%;border:0px;border-spacing:0px;border-collapse:separate;margin-right:auto;margin-left:auto;"><tbody>
<tr>
<td style="padding:0px">
<br>
<p style="text-align:right;font-size:small;">
<a href="https://github.com/jonbarron/jonbarron_website">Source code</a>
was developed by Jon Barron.
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