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I got this error while executing Main_Doublet_Decon function with a particular Seurat Object. Changing the Seurat Object (another dataset) removed the error. Any clues? This is how I proceeded from the Seurat object:
I got this error while executing Main_Doublet_Decon function with a particular Seurat Object. Changing the Seurat Object (another dataset) removed the error. Any clues? This is how I proceeded from the Seurat object:
new.file=Improved_Seurat_Pre_Process(Seuratobj, num_genes=50, write_files=FALSE)
filename="newFile"
location="/Volumes/Samsung_T5/"
write.table(new.file$newExpressionFile, paste0(location=location, filename=filename, "_expression"), sep="\t")
write.table(new.file$newFullExpressionFile, paste0(location=location, filename=filename, "_fullExpression"), sep="\t")
write.table(new.file$newGroupsFile, paste0(location=location, filename=filename, "_groups"), sep="\t", col.names = F)
res.new.file=Main_Doublet_Decon(rawDataFile=new.file$newExpressionFile,
groupsFile=new.file$newGroupsFile,
filename=filename,
location=location,
fullDataFile=NULL,
removeCC=FALSE,
species="hsa",
rhop=1.1,
write=TRUE,
PMF=TRUE,
useFull=FALSE,
heatmap=FALSE,
centroids=TRUE,
num_doubs=100,
only50=FALSE,
min_uniq=4,
nCores=-1)
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